TY - JOUR
T1 - Localization and characterization of the hippuricase activity of group B streptococci
AU - Ferrieri, Patricia
AU - Wannamaker, L. W.
AU - Nelson, J.
PY - 1973/12/1
Y1 - 1973/12/1
N2 - Studies are presented on the isolation, localization, and characterization of hippuricase activity of group B streptococci. Washed, intact cells, live or heat killed at 56 C, exhibited hydrolysis of hippuric acid, but cell free filtrates of the organism did not. Excellent hippuricase activity was recoverable from supernatant fluids of mechanically disrupted cells, and evidence suggests that it exists largely intracellularly. Characteristics of the hippuricase preparation are consistent with the view that the biologically active principle is an enzyme. A quantitative microtiter technique has been developed which is useful in titrating enzymatic activity and antibody neutralization. Sera from rabbits immunized with filtered preparations neutralized hippuricase activity.
AB - Studies are presented on the isolation, localization, and characterization of hippuricase activity of group B streptococci. Washed, intact cells, live or heat killed at 56 C, exhibited hydrolysis of hippuric acid, but cell free filtrates of the organism did not. Excellent hippuricase activity was recoverable from supernatant fluids of mechanically disrupted cells, and evidence suggests that it exists largely intracellularly. Characteristics of the hippuricase preparation are consistent with the view that the biologically active principle is an enzyme. A quantitative microtiter technique has been developed which is useful in titrating enzymatic activity and antibody neutralization. Sera from rabbits immunized with filtered preparations neutralized hippuricase activity.
UR - http://www.scopus.com/inward/record.url?scp=0015789281&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0015789281&partnerID=8YFLogxK
M3 - Article
C2 - 4797183
AN - SCOPUS:0015789281
SN - 0019-9567
VL - 7
SP - 747
EP - 752
JO - Infection and immunity
JF - Infection and immunity
IS - 5
ER -