Magnetic ionic liquids as PCR-compatible solvents for DNA extraction from biological samples

Kevin D. Clark, Melissa M. Yamsek, Omprakash Nacham, Jared L. Anderson

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

A polymerase chain reaction (PCR) buffer was systematically designed to relieve the inhibition caused by hydrophobic magnetic ionic liquids (MILs). We describe a simple, rapid method for MIL-based plasmid DNA extraction from crude bacterial cell lysate in which DNA-enriched MIL is transferred directly to a PCR tube for analysis.

Original languageEnglish (US)
Pages (from-to)16771-16773
Number of pages3
JournalChemical Communications
Volume51
Issue number94
DOIs
StatePublished - Sep 29 2015

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