Magnetic ionic liquids as PCR-compatible solvents for DNA extraction from biological samples

Kevin D. Clark; Melissa M. Yamsek; Omprakash Nacham; Jared L. Anderson

doi:10.1039/c5cc07253k

Magnetic ionic liquids as PCR-compatible solvents for DNA extraction from biological samples

Kevin D. Clark, Melissa M. Yamsek, Omprakash Nacham, Jared L. Anderson

Chemistry (Twin Cities)

Research output: Contribution to journal › Article › peer-review

70 Scopus citations

Abstract

A polymerase chain reaction (PCR) buffer was systematically designed to relieve the inhibition caused by hydrophobic magnetic ionic liquids (MILs). We describe a simple, rapid method for MIL-based plasmid DNA extraction from crude bacterial cell lysate in which DNA-enriched MIL is transferred directly to a PCR tube for analysis.

Original language	English (US)
Pages (from-to)	16771-16773
Number of pages	3
Journal	Chemical Communications
Volume	51
Issue number	94
DOIs	https://doi.org/10.1039/c5cc07253k
State	Published - Sep 29 2015

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© The Royal Society of Chemistry.

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title = "Magnetic ionic liquids as PCR-compatible solvents for DNA extraction from biological samples",

abstract = "A polymerase chain reaction (PCR) buffer was systematically designed to relieve the inhibition caused by hydrophobic magnetic ionic liquids (MILs). We describe a simple, rapid method for MIL-based plasmid DNA extraction from crude bacterial cell lysate in which DNA-enriched MIL is transferred directly to a PCR tube for analysis.",

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AU - Nacham, Omprakash

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AB - A polymerase chain reaction (PCR) buffer was systematically designed to relieve the inhibition caused by hydrophobic magnetic ionic liquids (MILs). We describe a simple, rapid method for MIL-based plasmid DNA extraction from crude bacterial cell lysate in which DNA-enriched MIL is transferred directly to a PCR tube for analysis.

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Magnetic ionic liquids as PCR-compatible solvents for DNA extraction from biological samples

Abstract

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