TY - JOUR
T1 - Maize inbreds exhibit high levels of copy number variation (CNV) and presence/absence variation (PAV) in genome content
AU - Springer, Nathan M.
AU - Ying, Kai
AU - Fu, Yan
AU - Ji, Tieming
AU - Yeh, Cheng Ting
AU - Jia, Yi
AU - Wu, Wei
AU - Richmond, Todd
AU - Kitzman, Jacob
AU - Rosenbaum, Heidi
AU - Iniguez, A. Leonardo
AU - Barbazuk, W. Brad
AU - Jeddeloh, Jeffrey A.
AU - Nettleton, Dan
AU - Schnable, Patrick S.
PY - 2009/11
Y1 - 2009/11
N2 - Following the domestication of maize over the past ∼10,000 years, breeders have exploited the extensive genetic diversity of this species to mold its phenotype to meet human needs. The extent of structural variation, including copy number variation (CNV) and presence/absence variation (PAV), which are thought to contribute to the extraordinary phenotypic diversity and plasticity of this important crop, have not been elucidated. Whole-genome, array-based, comparative genomic hybridization (CGH) revealed a level of structural diversity between the inbred lines B73 and Mo17 that is unprecedented among higher eukaryotes. A detailed analysis of altered segments of DNA conservatively estimates that there are several hundred CNV sequences among the two genotypes, as well as several thousand PAV sequences that are present in B73 but not Mo17. Haplotype-specific PAVs contain hundreds of single-copy, expressed genes that may contribute to heterosis and to the extraordinary phenotypic diversity of this important crop.
AB - Following the domestication of maize over the past ∼10,000 years, breeders have exploited the extensive genetic diversity of this species to mold its phenotype to meet human needs. The extent of structural variation, including copy number variation (CNV) and presence/absence variation (PAV), which are thought to contribute to the extraordinary phenotypic diversity and plasticity of this important crop, have not been elucidated. Whole-genome, array-based, comparative genomic hybridization (CGH) revealed a level of structural diversity between the inbred lines B73 and Mo17 that is unprecedented among higher eukaryotes. A detailed analysis of altered segments of DNA conservatively estimates that there are several hundred CNV sequences among the two genotypes, as well as several thousand PAV sequences that are present in B73 but not Mo17. Haplotype-specific PAVs contain hundreds of single-copy, expressed genes that may contribute to heterosis and to the extraordinary phenotypic diversity of this important crop.
UR - http://www.scopus.com/inward/record.url?scp=73649121258&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=73649121258&partnerID=8YFLogxK
U2 - 10.1371/journal.pgen.1000734
DO - 10.1371/journal.pgen.1000734
M3 - Article
C2 - 19956538
AN - SCOPUS:73649121258
SN - 1553-7390
VL - 5
JO - PLoS genetics
JF - PLoS genetics
IS - 11
M1 - e1000734
ER -
