Measurement of cyclic nucleotides in histologically defined samples of brain and retina

J. A. Ferrendelli; E. H. Rubin; H. T. Orr; D. A. Kinscherf; O. H. Lowry

doi:10.1016/0003-2697(77)90030-6

Measurement of cyclic nucleotides in histologically defined samples of brain and retina

J. A. Ferrendelli, E. H. Rubin, H. T. Orr, D. A. Kinscherf, O. H. Lowry

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

A method is described which allows measurement of cyclic AMP and cyclic GMP in histologically defined tissue samples containing as little as 0.6 fmol of either compound. This permits, for example, assays of freeze-dried samples as small as 0.03 μg dry weight from individual retina layers or 4-μg samples from individual layers of cerebellum. The procedure involves a modified radioimmunoassay at reduced volume for acetylated cyclic nucleotides in combination with some of the techniques previously developed for quantitative histochemical assays.

Original language	English (US)
Pages (from-to)	252-259
Number of pages	8
Journal	Analytical Biochemistry
Volume	78
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(77)90030-6
State	Published - Mar 1977
Externally published	Yes

Bibliographical note

Funding Information:
by USPHS Grants NS-09667, NS-13104, NS-08862, NS-05613, Cancer Society Grant BC-4Q. should be addressed.

Access

10.1016/0003-2697(77)90030-6

OpenUrl availability

Full text

Cite this

@article{89ad6ff216964c8ca8ff9cc2c82a1237,

title = "Measurement of cyclic nucleotides in histologically defined samples of brain and retina",

abstract = "A method is described which allows measurement of cyclic AMP and cyclic GMP in histologically defined tissue samples containing as little as 0.6 fmol of either compound. This permits, for example, assays of freeze-dried samples as small as 0.03 μg dry weight from individual retina layers or 4-μg samples from individual layers of cerebellum. The procedure involves a modified radioimmunoassay at reduced volume for acetylated cyclic nucleotides in combination with some of the techniques previously developed for quantitative histochemical assays.",

author = "Ferrendelli, {J. A.} and Rubin, {E. H.} and Orr, {H. T.} and Kinscherf, {D. A.} and Lowry, {O. H.}",

note = "Funding Information: by USPHS Grants NS-09667, NS-13104, NS-08862, NS-05613, Cancer Society Grant BC-4Q. should be addressed.",

year = "1977",

month = mar,

doi = "10.1016/0003-2697(77)90030-6",

language = "English (US)",

volume = "78",

pages = "252--259",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Measurement of cyclic nucleotides in histologically defined samples of brain and retina

AU - Ferrendelli, J. A.

AU - Rubin, E. H.

AU - Orr, H. T.

AU - Kinscherf, D. A.

AU - Lowry, O. H.

N1 - Funding Information: by USPHS Grants NS-09667, NS-13104, NS-08862, NS-05613, Cancer Society Grant BC-4Q. should be addressed.

PY - 1977/3

Y1 - 1977/3

N2 - A method is described which allows measurement of cyclic AMP and cyclic GMP in histologically defined tissue samples containing as little as 0.6 fmol of either compound. This permits, for example, assays of freeze-dried samples as small as 0.03 μg dry weight from individual retina layers or 4-μg samples from individual layers of cerebellum. The procedure involves a modified radioimmunoassay at reduced volume for acetylated cyclic nucleotides in combination with some of the techniques previously developed for quantitative histochemical assays.

AB - A method is described which allows measurement of cyclic AMP and cyclic GMP in histologically defined tissue samples containing as little as 0.6 fmol of either compound. This permits, for example, assays of freeze-dried samples as small as 0.03 μg dry weight from individual retina layers or 4-μg samples from individual layers of cerebellum. The procedure involves a modified radioimmunoassay at reduced volume for acetylated cyclic nucleotides in combination with some of the techniques previously developed for quantitative histochemical assays.

UR - http://www.scopus.com/inward/record.url?scp=0017349289&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017349289&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(77)90030-6

DO - 10.1016/0003-2697(77)90030-6

M3 - Article

C2 - 192102

AN - SCOPUS:0017349289

SN - 0003-2697

VL - 78

SP - 252

EP - 259

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

Measurement of cyclic nucleotides in histologically defined samples of brain and retina

Abstract

Bibliographical note

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this