Mechanism of induction of cytochrome P-450ac (P-450j) in chemically induced and spontaneously diabetic rats

Zigong Dong; Junyan Hong; Qiang Ma; Dechun Li; John Bullock; Frank J. Gonzalez; Sang S. Park; Harry V. Gelboin; Chung S. Yang

doi:10.1016/0003-9861(88)90610-8

Mechanism of induction of cytochrome P-450_ac (P-450_j) in chemically induced and spontaneously diabetic rats

Zigong Dong, Junyan Hong, Qiang Ma, Dechun Li, John Bullock, Frank J. Gonzalez, Sang S. Park, Harry V. Gelboin, Chung S. Yang

Hormel Institute

Research output: Contribution to journal › Article › peer-review

149 Scopus citations

Abstract

Previous studies demonstrated that a microsomal high-affinity N-nitrosodimethylamine demethylase activity and cytochrome P-450_ac (an acetone/ethanol-inducible form) were induced by streptozotocin-induced diabetes in rats. In the present work, the induction was studied in detail in two chemically induced (by streptozotocin and alloxan) diabetic rat models and one spontaneously ( BB Wor) diabetic rat model. All the diabetic conditions caused increases in three parameters: (a) microsomal N-nitrosodimethylamine demethylase activity which is known to be a good indicator of the level of P-450_ac; (b) the levels of P-450_ac as determined by immunoblot analysis; and (c) the levels of mRNA of P-450_ac as determined by hybridization assays with a cDNA probe for this enzyme. These increases were abolished by treatment of the diabetic rats with insulin. The results suggest that the pathophysiological condition of diabetes is responsible for the induction of P-450_ac and elevation of mRNA is involved in all of the three diabetic models investigated.

Original language	English (US)
Pages (from-to)	29-35
Number of pages	7
Journal	Archives of Biochemistry and Biophysics
Volume	263
Issue number	1
DOIs	https://doi.org/10.1016/0003-9861(88)90610-8
State	Published - May 15 1988

Bibliographical note

Funding Information:
1 This work was supported ES-03938 from the NIH. ‘To whom correspondence

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title = "Mechanism of induction of cytochrome P-450ac (P-450j) in chemically induced and spontaneously diabetic rats",

abstract = "Previous studies demonstrated that a microsomal high-affinity N-nitrosodimethylamine demethylase activity and cytochrome P-450ac (an acetone/ethanol-inducible form) were induced by streptozotocin-induced diabetes in rats. In the present work, the induction was studied in detail in two chemically induced (by streptozotocin and alloxan) diabetic rat models and one spontaneously ( BB Wor) diabetic rat model. All the diabetic conditions caused increases in three parameters: (a) microsomal N-nitrosodimethylamine demethylase activity which is known to be a good indicator of the level of P-450ac; (b) the levels of P-450ac as determined by immunoblot analysis; and (c) the levels of mRNA of P-450ac as determined by hybridization assays with a cDNA probe for this enzyme. These increases were abolished by treatment of the diabetic rats with insulin. The results suggest that the pathophysiological condition of diabetes is responsible for the induction of P-450ac and elevation of mRNA is involved in all of the three diabetic models investigated.",

author = "Zigong Dong and Junyan Hong and Qiang Ma and Dechun Li and John Bullock and Gonzalez, {Frank J.} and Park, {Sang S.} and Gelboin, {Harry V.} and Yang, {Chung S.}",

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AU - Dong, Zigong

AU - Hong, Junyan

AU - Ma, Qiang

AU - Li, Dechun

AU - Bullock, John

AU - Gonzalez, Frank J.

AU - Park, Sang S.

AU - Gelboin, Harry V.

AU - Yang, Chung S.

N1 - Funding Information: 1 This work was supported ES-03938 from the NIH. ‘To whom correspondence

PY - 1988/5/15

Y1 - 1988/5/15

N2 - Previous studies demonstrated that a microsomal high-affinity N-nitrosodimethylamine demethylase activity and cytochrome P-450ac (an acetone/ethanol-inducible form) were induced by streptozotocin-induced diabetes in rats. In the present work, the induction was studied in detail in two chemically induced (by streptozotocin and alloxan) diabetic rat models and one spontaneously ( BB Wor) diabetic rat model. All the diabetic conditions caused increases in three parameters: (a) microsomal N-nitrosodimethylamine demethylase activity which is known to be a good indicator of the level of P-450ac; (b) the levels of P-450ac as determined by immunoblot analysis; and (c) the levels of mRNA of P-450ac as determined by hybridization assays with a cDNA probe for this enzyme. These increases were abolished by treatment of the diabetic rats with insulin. The results suggest that the pathophysiological condition of diabetes is responsible for the induction of P-450ac and elevation of mRNA is involved in all of the three diabetic models investigated.

AB - Previous studies demonstrated that a microsomal high-affinity N-nitrosodimethylamine demethylase activity and cytochrome P-450ac (an acetone/ethanol-inducible form) were induced by streptozotocin-induced diabetes in rats. In the present work, the induction was studied in detail in two chemically induced (by streptozotocin and alloxan) diabetic rat models and one spontaneously ( BB Wor) diabetic rat model. All the diabetic conditions caused increases in three parameters: (a) microsomal N-nitrosodimethylamine demethylase activity which is known to be a good indicator of the level of P-450ac; (b) the levels of P-450ac as determined by immunoblot analysis; and (c) the levels of mRNA of P-450ac as determined by hybridization assays with a cDNA probe for this enzyme. These increases were abolished by treatment of the diabetic rats with insulin. The results suggest that the pathophysiological condition of diabetes is responsible for the induction of P-450ac and elevation of mRNA is involved in all of the three diabetic models investigated.

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