Mediation of neurogenic ion transport by acetylcholine, prostanoids and 5-hydroxytryptamine in porcine ileum

DeWayne Townsend; Melissa A. Casey; David R Brown

doi:10.1016/j.ejphar.2005.07.023

Mediation of neurogenic ion transport by acetylcholine, prostanoids and 5-hydroxytryptamine in porcine ileum

DeWayne Townsend, Melissa A. Casey, David R Brown

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7 Scopus citations

Abstract

Enteric neural activity modulates active transepithelial ion transport in the intestine. We investigated the neural circuits mediating neurogenic secretion in mucosal explants from porcine ileum. Transmural electrical stimulation increased short-circuit current, a measure of active ion transport, by 35 ± 2 μA/cm². The neuronal Na⁺ channel blocker saxitoxin, the muscarinic cholinergic receptor antagonist atropine, the 5-hydroxytryptamine₃ receptor antagonist tropisetron, and the cyclooxygenase inhibitor indomethacin inhibited this response. In addition, tropisetron inhibited the atropine-resistant portion of the response, and both atropine and indomethacin attenuated the saxitoxin-resistant component. Neurogenic secretion in porcine ileum appears to be mediated by tryptaminergic and prostanoid-sensitive cholinergic pathways.

Original language	English (US)
Pages (from-to)	285-289
Number of pages	5
Journal	European Journal of Pharmacology
Volume	519
Issue number	3
DOIs	https://doi.org/10.1016/j.ejphar.2005.07.023
State	Published - Sep 20 2005

Bibliographical note

Funding Information:
This investigation was supported in part by National Institutes of Health grants R01 DA-10200 and T32 DA-07234.

Keywords

5-hydroxytryptamine receptor
Intestinal mucosa
Muscarinic cholinergic receptor
Short-circuit current
Transmural electrical stimulation

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title = "Mediation of neurogenic ion transport by acetylcholine, prostanoids and 5-hydroxytryptamine in porcine ileum",

abstract = "Enteric neural activity modulates active transepithelial ion transport in the intestine. We investigated the neural circuits mediating neurogenic secretion in mucosal explants from porcine ileum. Transmural electrical stimulation increased short-circuit current, a measure of active ion transport, by 35 ± 2 μA/cm2. The neuronal Na+ channel blocker saxitoxin, the muscarinic cholinergic receptor antagonist atropine, the 5-hydroxytryptamine3 receptor antagonist tropisetron, and the cyclooxygenase inhibitor indomethacin inhibited this response. In addition, tropisetron inhibited the atropine-resistant portion of the response, and both atropine and indomethacin attenuated the saxitoxin-resistant component. Neurogenic secretion in porcine ileum appears to be mediated by tryptaminergic and prostanoid-sensitive cholinergic pathways.",

keywords = "5-hydroxytryptamine receptor, Intestinal mucosa, Muscarinic cholinergic receptor, Short-circuit current, Transmural electrical stimulation",

author = "DeWayne Townsend and Casey, {Melissa A.} and Brown, {David R}",

note = "Funding Information: This investigation was supported in part by National Institutes of Health grants R01 DA-10200 and T32 DA-07234.",

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AU - Townsend, DeWayne

AU - Casey, Melissa A.

AU - Brown, David R

N1 - Funding Information: This investigation was supported in part by National Institutes of Health grants R01 DA-10200 and T32 DA-07234.

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N2 - Enteric neural activity modulates active transepithelial ion transport in the intestine. We investigated the neural circuits mediating neurogenic secretion in mucosal explants from porcine ileum. Transmural electrical stimulation increased short-circuit current, a measure of active ion transport, by 35 ± 2 μA/cm2. The neuronal Na+ channel blocker saxitoxin, the muscarinic cholinergic receptor antagonist atropine, the 5-hydroxytryptamine3 receptor antagonist tropisetron, and the cyclooxygenase inhibitor indomethacin inhibited this response. In addition, tropisetron inhibited the atropine-resistant portion of the response, and both atropine and indomethacin attenuated the saxitoxin-resistant component. Neurogenic secretion in porcine ileum appears to be mediated by tryptaminergic and prostanoid-sensitive cholinergic pathways.

AB - Enteric neural activity modulates active transepithelial ion transport in the intestine. We investigated the neural circuits mediating neurogenic secretion in mucosal explants from porcine ileum. Transmural electrical stimulation increased short-circuit current, a measure of active ion transport, by 35 ± 2 μA/cm2. The neuronal Na+ channel blocker saxitoxin, the muscarinic cholinergic receptor antagonist atropine, the 5-hydroxytryptamine3 receptor antagonist tropisetron, and the cyclooxygenase inhibitor indomethacin inhibited this response. In addition, tropisetron inhibited the atropine-resistant portion of the response, and both atropine and indomethacin attenuated the saxitoxin-resistant component. Neurogenic secretion in porcine ileum appears to be mediated by tryptaminergic and prostanoid-sensitive cholinergic pathways.

KW - 5-hydroxytryptamine receptor

KW - Intestinal mucosa

KW - Muscarinic cholinergic receptor

KW - Short-circuit current

KW - Transmural electrical stimulation

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Mediation of neurogenic ion transport by acetylcholine, prostanoids and 5-hydroxytryptamine in porcine ileum

Abstract

Bibliographical note

Keywords

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