Abstract
Metabolomics is an “omics” approach to quantitatively measure a large set of metabolites. In this chapter, we describe an example method for performing liquid chromatography coupled to mass spectrometry (LC–MS)-based untargeted metabolomics on a cell extract from Vibrio cholerae.
Original language | English (US) |
---|---|
Title of host publication | Methods in Molecular Biology |
Publisher | Humana Press Inc. |
Pages | 45-51 |
Number of pages | 7 |
DOIs | |
State | Published - 2018 |
Publication series
Name | Methods in Molecular Biology |
---|---|
Volume | 1839 |
ISSN (Print) | 1064-3745 |
Bibliographical note
Funding Information:We thank Dr. Fred Stevens for helpful discussions. This research was supported by a grant from the National Institutes of Health to C.C.H. [AI-063121-02].
Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2018.
Keywords
- Liquid chromatography–mass spectrometry (LC–MS)
- Phenyl-bonded phase
- Q-TOF mass spectrometer
- Reversed-phase chromatography
- Untargeted metabolomics