Metabolomics of vibrio cholerae

Yusuke Minato, Jay S. Kirkwood, Claudia C. Häse

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Metabolomics is an “omics” approach to quantitatively measure a large set of metabolites. In this chapter, we describe an example method for performing liquid chromatography coupled to mass spectrometry (LC–MS)-based untargeted metabolomics on a cell extract from Vibrio cholerae.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages45-51
Number of pages7
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1839
ISSN (Print)1064-3745

Bibliographical note

Funding Information:
We thank Dr. Fred Stevens for helpful discussions. This research was supported by a grant from the National Institutes of Health to C.C.H. [AI-063121-02].

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2018.

Keywords

  • Liquid chromatography–mass spectrometry (LC–MS)
  • Phenyl-bonded phase
  • Q-TOF mass spectrometer
  • Reversed-phase chromatography
  • Untargeted metabolomics

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