Metabolomics is an “omics” approach to quantitatively measure a large set of metabolites. In this chapter, we describe an example method for performing liquid chromatography coupled to mass spectrometry (LC–MS)-based untargeted metabolomics on a cell extract from Vibrio cholerae.
|Original language||English (US)|
|Title of host publication||Methods in Molecular Biology|
|Publisher||Humana Press Inc.|
|Number of pages||7|
|State||Published - 2018|
|Name||Methods in Molecular Biology|
Bibliographical noteFunding Information:
We thank Dr. Fred Stevens for helpful discussions. This research was supported by a grant from the National Institutes of Health to C.C.H. [AI-063121-02].
© Springer Science+Business Media, LLC, part of Springer Nature 2018.
- Liquid chromatography–mass spectrometry (LC–MS)
- Phenyl-bonded phase
- Q-TOF mass spectrometer
- Reversed-phase chromatography
- Untargeted metabolomics