Microchip electrophoresis devices for the detection of nitric oxide: Comparison of bulk cell and single cell analysis

Susan M. Lunte, Dulan B. Gunasekara, Eve C. Metto, Matthew K. Hulvey, Emilie R. Mainz, Giuseppe Caruso, Jose Alberto Fracassi Da Silva, Derek T. Jensen, Anne H. Culbertson, Ryan J. Grigsby, Christopher T. Culbertson

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

This paper describes the development of microchip electrophoresis (ME) devices for detection of nitric oxide (NO) from bulk cell lysates and single cells. Detection of NO and other endogenous electroactive species in Jurkat cells was achieved using ME with amperometric detection. Electrochemical (EC) results were then validated by ME coupled to laser-induced fluorescence (LIF) using 4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate (DAF-FMDA), a NO-specific probe. In parallel, a single cell analysis device was developed for the determination of intracellular NO using DAF-FMDA and LIF detection.

Original languageEnglish (US)
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1728-1730
Number of pages3
StatePublished - Dec 1 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume3

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period10/2/1110/6/11

Keywords

  • Amperometric detection
  • Bulk cell analysis
  • Laser induced fluorescence detection
  • Microchip electrophoresis
  • Nitric oxide
  • Single cell analysis

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