Abstract
Lake trout, Salvelinus namaycush, is one of the few salmonids with morphologically differentiated sex chromosomes. Genetic analysis suggested that the sex-determining region of this species lies on the short arm of the Y chromosome. The differential arm of the Y chromosome was microdissected and the resulting DNA amplified in a sequence-independent manner. Amplified DNA was biotin labeled as a probe for fluorescence in situ hybridization (FISH). Strong hybridization signals were seen covering defined regions of both the Y and X chromosomes. Homeologous chromosomes of the ancestrally tetraploid genome were not identified by FISH with the Y probe, indicating diploidization of this region of the genome.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 221-226 |
| Number of pages | 6 |
| Journal | Chromosome Research |
| Volume | 3 |
| Issue number | 4 |
| DOIs | |
| State | Published - Jun 1995 |
Keywords
- fluorescence in situ hybridization
- lake trout
- microdissection
- sex chromosomes