Systèmes microfluidiques de particules magnétiques auto-assemblées; application à la séparation d'ADN et à la digestion de protéines

Nicolas Minc; Marcela Slovakova; Kevin D. Dorfman; Claus Fütterer; Plamen Bokov; Zuzana Bilkova; Claire Smadga; Myriam Taverna; Jean Louis Viovy

doi:10.1051/lhb:200604007

Systèmes microfluidiques de particules magnétiques auto-assemblées; application à la séparation d'ADN et à la digestion de protéines

Translated title of the contribution: Microfluidic systems of self-assembled magnetic particles; application to DNA separation and protein digestion

Nicolas Minc, Marcela Slovakova, Kevin D. Dorfman, Claus Fütterer, Plamen Bokov, Zuzana Bilkova, Claire Smadga, Myriam Taverna, Jean Louis Viovy

Chemical Engineering and Materials Science

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

We present two experiments of bioanalysis based on the self-organization of magnetic beads under a magnetic field, realized microfluidic chips, In the first one, the beads are organized in a quasi-hexagonal array of columns used as a matrix to achieve and study the separation of long fragments of DNA. In the second one, trypsin is covalently grafted onto beads that are immobilized in a microchannel, and different proteins are then digested through the bead plug. The products of digestion are then analyzed hy MS or CE.

Translated title of the contribution	Microfluidic systems of self-assembled magnetic particles; application to DNA separation and protein digestion
Original language	French
Pages (from-to)	51-54
Number of pages	4
Journal	Houille Blanche
Issue number	4
DOIs	https://doi.org/10.1051/lhb:200604007
State	Published - 2006

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title = "Syst{\`e}mes microfluidiques de particules magn{\'e}tiques auto-assembl{\'e}es; application {\`a} la s{\'e}paration d'ADN et {\`a} la digestion de prot{\'e}ines",

abstract = "We present two experiments of bioanalysis based on the self-organization of magnetic beads under a magnetic field, realized microfluidic chips, In the first one, the beads are organized in a quasi-hexagonal array of columns used as a matrix to achieve and study the separation of long fragments of DNA. In the second one, trypsin is covalently grafted onto beads that are immobilized in a microchannel, and different proteins are then digested through the bead plug. The products of digestion are then analyzed hy MS or CE.",

author = "Nicolas Minc and Marcela Slovakova and Dorfman, {Kevin D.} and Claus F{\"u}tterer and Plamen Bokov and Zuzana Bilkova and Claire Smadga and Myriam Taverna and Viovy, {Jean Louis}",

year = "2006",

doi = "10.1051/lhb:200604007",

language = "French",

pages = "51--54",

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T1 - Systèmes microfluidiques de particules magnétiques auto-assemblées; application à la séparation d'ADN et à la digestion de protéines

AU - Minc, Nicolas

AU - Slovakova, Marcela

AU - Dorfman, Kevin D.

AU - Fütterer, Claus

AU - Bokov, Plamen

AU - Bilkova, Zuzana

AU - Smadga, Claire

AU - Taverna, Myriam

AU - Viovy, Jean Louis

PY - 2006

Y1 - 2006

N2 - We present two experiments of bioanalysis based on the self-organization of magnetic beads under a magnetic field, realized microfluidic chips, In the first one, the beads are organized in a quasi-hexagonal array of columns used as a matrix to achieve and study the separation of long fragments of DNA. In the second one, trypsin is covalently grafted onto beads that are immobilized in a microchannel, and different proteins are then digested through the bead plug. The products of digestion are then analyzed hy MS or CE.

AB - We present two experiments of bioanalysis based on the self-organization of magnetic beads under a magnetic field, realized microfluidic chips, In the first one, the beads are organized in a quasi-hexagonal array of columns used as a matrix to achieve and study the separation of long fragments of DNA. In the second one, trypsin is covalently grafted onto beads that are immobilized in a microchannel, and different proteins are then digested through the bead plug. The products of digestion are then analyzed hy MS or CE.

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JF - Houille Blanche

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Systèmes microfluidiques de particules magnétiques auto-assemblées; application à la séparation d'ADN et à la digestion de protéines

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