TY - JOUR
T1 - Microglial response to amyloid plaques in APPsw transgenic mice
AU - Frautschy, Sally A.
AU - Yang, Fusheng
AU - Irrizarry, Michael
AU - Hyman, Brad
AU - Saido, T. C.
AU - Hsiao, Karen
AU - Cole, Greg M.
PY - 1998/1
Y1 - 1998/1
N2 - Microglial activation is central to the inflammatory response in Alzheimer's Disease (AD). A recently described mouse line, Tg(HuAPP695.K670N/M671L)2576, expressing human amyloid precursor protein with a familial AD gene mutation, age-related amyloid deposits, and memory deficits, was found to develop a significant microglial response using Griffonia simplicifolia lectin or phosphotyrosine probe to identify microglia. Both Griffonia simplicifolia lectin and phosphotyrosine staining showed increased numbers of intensely labeled, often enlarged microglia clustered in and around plaques, consistent with microglial activation related to β-amyloid formation. Using quantitative image analysis of coronal phosphotyrosine-immunostained sections, transgene-positive 10- to 16-month- old, hemizygous, hybrid Tg2576 (APPsw) animals showed significantly increased microglial density and size in plaque-forming areas of hippocampus and frontal, entorhinal, and occipital cortex. Quantitative analysis of microglia as a function of distance from the center of plaques (double labeled for Aβ peptide and microglia) revealed highly significant, two- to fivefold elevations in microglial number and area within plaques compared with neighboring regions. Tg2576 β-amyloid-plaque-forming mice should be a useful system for assessing the consequences of the microglial-mediated inflammatory response to β-amyloid and developing anti-inflammatory therapeutic strategies for Alzheimer's disease. These results provide the first quantitative link between β-amyloid plaque formation and microglial activation in an animal model with neuritic plaques and memory deficits.
AB - Microglial activation is central to the inflammatory response in Alzheimer's Disease (AD). A recently described mouse line, Tg(HuAPP695.K670N/M671L)2576, expressing human amyloid precursor protein with a familial AD gene mutation, age-related amyloid deposits, and memory deficits, was found to develop a significant microglial response using Griffonia simplicifolia lectin or phosphotyrosine probe to identify microglia. Both Griffonia simplicifolia lectin and phosphotyrosine staining showed increased numbers of intensely labeled, often enlarged microglia clustered in and around plaques, consistent with microglial activation related to β-amyloid formation. Using quantitative image analysis of coronal phosphotyrosine-immunostained sections, transgene-positive 10- to 16-month- old, hemizygous, hybrid Tg2576 (APPsw) animals showed significantly increased microglial density and size in plaque-forming areas of hippocampus and frontal, entorhinal, and occipital cortex. Quantitative analysis of microglia as a function of distance from the center of plaques (double labeled for Aβ peptide and microglia) revealed highly significant, two- to fivefold elevations in microglial number and area within plaques compared with neighboring regions. Tg2576 β-amyloid-plaque-forming mice should be a useful system for assessing the consequences of the microglial-mediated inflammatory response to β-amyloid and developing anti-inflammatory therapeutic strategies for Alzheimer's disease. These results provide the first quantitative link between β-amyloid plaque formation and microglial activation in an animal model with neuritic plaques and memory deficits.
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M3 - Article
C2 - 9422548
AN - SCOPUS:0031974406
SN - 0002-9440
VL - 152
SP - 307
EP - 317
JO - American Journal of Pathology
JF - American Journal of Pathology
IS - 1
ER -