Modulation of Cytokine mRNA Expression in Pharyngeal Epithelial Samples obtained from Cattle Infected with Foot-and-Mouth Disease Virus

C. Stenfeldt; P. M H Heegaard; A. Stockmarr; G. J. Belsham

doi:10.1016/j.jcpa.2011.06.005

Modulation of Cytokine mRNA Expression in Pharyngeal Epithelial Samples obtained from Cattle Infected with Foot-and-Mouth Disease Virus

C. Stenfeldt, P. M H Heegaard, A. Stockmarr, G. J. Belsham

Veterinary Population Medicine

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

A novel technique of endoscopical collection of small tissue samples was used to obtain sequential tissue samples from the dorsal soft palate (DSP) of individual cattle infected with foot-and-mouth disease virus (FMDV) at different phases of the infection. Levels of mRNA encoding interferon (IFN)-α and IFN-β as well as tumour necrosis factor (TNF)-α were measured in these samples by quantitative reverse transcriptase polymerase chain reaction. Expression of IFN-β mRNA was significantly down-regulated in the biopsy samples harvested during the acute phase of infection, while there was no statistically significant effect on the expression of IFN-α mRNA compared with baseline levels. In contrast, the mRNA encoding TNF-α was significantly up-regulated in samples collected during both acute and late (>28. days post infection) phases of infection. There were also significantly higher levels of TNF-α mRNA expressed in samples derived from animals that were identified subsequently as persistently infected FMDV-carriers. It was concluded that there was a significant difference in the host-response in the DSP of calves that were identified as persistently infected, subclinical carriers of FMDV.

Original language	English (US)
Pages (from-to)	243-252
Number of pages	10
Journal	Journal of Comparative Pathology
Volume	146
Issue number	2-3
DOIs	https://doi.org/10.1016/j.jcpa.2011.06.005
State	Published - Feb 2012

Bibliographical note

Funding Information:
This work was financed in part by a PhD scholarship to CS funded by the Technical University of Denmark (DTU) and the Research School for Animal Production and Health at the Faculty of Life Sciences , Copenhagen University. Dr S. Alexandersen, CFIA-NCAD, Winnipeg, Canada, is acknowledged for having supplied the original virus inoculum. J. Bashiruddin, IAH-Pirbright is thanked for having assisted with experimental protocols for the gene-expression analysis. K. Tjørnehøj is thanked for assistance during performance of animal experiments. Animal care-takers H. Andersen, J. Oxfeldt, H. Lehman, O. Bille and M. Petersen are thanked for their assistance. P. Normann, J. Borch, J. Christiansen, J. Jensen, T. Rasmussen and T. Frederiksen are thanked for excellent technical assistance with the analysis of samples.

Keywords

Cytokine mRNA
Foot-and-mouth disease
Pharyngeal epithelium

Access

10.1016/j.jcpa.2011.06.005

OpenUrl availability

Full text

Cite this

@article{b3c41ef501224dd7a8afe86c600adab3,

title = "Modulation of Cytokine mRNA Expression in Pharyngeal Epithelial Samples obtained from Cattle Infected with Foot-and-Mouth Disease Virus",

abstract = "A novel technique of endoscopical collection of small tissue samples was used to obtain sequential tissue samples from the dorsal soft palate (DSP) of individual cattle infected with foot-and-mouth disease virus (FMDV) at different phases of the infection. Levels of mRNA encoding interferon (IFN)-α and IFN-β as well as tumour necrosis factor (TNF)-α were measured in these samples by quantitative reverse transcriptase polymerase chain reaction. Expression of IFN-β mRNA was significantly down-regulated in the biopsy samples harvested during the acute phase of infection, while there was no statistically significant effect on the expression of IFN-α mRNA compared with baseline levels. In contrast, the mRNA encoding TNF-α was significantly up-regulated in samples collected during both acute and late (>28. days post infection) phases of infection. There were also significantly higher levels of TNF-α mRNA expressed in samples derived from animals that were identified subsequently as persistently infected FMDV-carriers. It was concluded that there was a significant difference in the host-response in the DSP of calves that were identified as persistently infected, subclinical carriers of FMDV.",

keywords = "Cytokine mRNA, Foot-and-mouth disease, Pharyngeal epithelium",

author = "C. Stenfeldt and Heegaard, {P. M H} and A. Stockmarr and Belsham, {G. J.}",

note = "Funding Information: This work was financed in part by a PhD scholarship to CS funded by the Technical University of Denmark (DTU) and the Research School for Animal Production and Health at the Faculty of Life Sciences , Copenhagen University. Dr S. Alexandersen, CFIA-NCAD, Winnipeg, Canada, is acknowledged for having supplied the original virus inoculum. J. Bashiruddin, IAH-Pirbright is thanked for having assisted with experimental protocols for the gene-expression analysis. K. Tj{\o}rneh{\o}j is thanked for assistance during performance of animal experiments. Animal care-takers H. Andersen, J. Oxfeldt, H. Lehman, O. Bille and M. Petersen are thanked for their assistance. P. Normann, J. Borch, J. Christiansen, J. Jensen, T. Rasmussen and T. Frederiksen are thanked for excellent technical assistance with the analysis of samples.",

year = "2012",

month = feb,

doi = "10.1016/j.jcpa.2011.06.005",

language = "English (US)",

volume = "146",

pages = "243--252",

journal = "Journal of Comparative Pathology",

issn = "0021-9975",

publisher = "W.B. Saunders Ltd",

number = "2-3",

}

TY - JOUR

T1 - Modulation of Cytokine mRNA Expression in Pharyngeal Epithelial Samples obtained from Cattle Infected with Foot-and-Mouth Disease Virus

AU - Stenfeldt, C.

AU - Heegaard, P. M H

AU - Stockmarr, A.

AU - Belsham, G. J.

N1 - Funding Information: This work was financed in part by a PhD scholarship to CS funded by the Technical University of Denmark (DTU) and the Research School for Animal Production and Health at the Faculty of Life Sciences , Copenhagen University. Dr S. Alexandersen, CFIA-NCAD, Winnipeg, Canada, is acknowledged for having supplied the original virus inoculum. J. Bashiruddin, IAH-Pirbright is thanked for having assisted with experimental protocols for the gene-expression analysis. K. Tjørnehøj is thanked for assistance during performance of animal experiments. Animal care-takers H. Andersen, J. Oxfeldt, H. Lehman, O. Bille and M. Petersen are thanked for their assistance. P. Normann, J. Borch, J. Christiansen, J. Jensen, T. Rasmussen and T. Frederiksen are thanked for excellent technical assistance with the analysis of samples.

PY - 2012/2

Y1 - 2012/2

N2 - A novel technique of endoscopical collection of small tissue samples was used to obtain sequential tissue samples from the dorsal soft palate (DSP) of individual cattle infected with foot-and-mouth disease virus (FMDV) at different phases of the infection. Levels of mRNA encoding interferon (IFN)-α and IFN-β as well as tumour necrosis factor (TNF)-α were measured in these samples by quantitative reverse transcriptase polymerase chain reaction. Expression of IFN-β mRNA was significantly down-regulated in the biopsy samples harvested during the acute phase of infection, while there was no statistically significant effect on the expression of IFN-α mRNA compared with baseline levels. In contrast, the mRNA encoding TNF-α was significantly up-regulated in samples collected during both acute and late (>28. days post infection) phases of infection. There were also significantly higher levels of TNF-α mRNA expressed in samples derived from animals that were identified subsequently as persistently infected FMDV-carriers. It was concluded that there was a significant difference in the host-response in the DSP of calves that were identified as persistently infected, subclinical carriers of FMDV.

AB - A novel technique of endoscopical collection of small tissue samples was used to obtain sequential tissue samples from the dorsal soft palate (DSP) of individual cattle infected with foot-and-mouth disease virus (FMDV) at different phases of the infection. Levels of mRNA encoding interferon (IFN)-α and IFN-β as well as tumour necrosis factor (TNF)-α were measured in these samples by quantitative reverse transcriptase polymerase chain reaction. Expression of IFN-β mRNA was significantly down-regulated in the biopsy samples harvested during the acute phase of infection, while there was no statistically significant effect on the expression of IFN-α mRNA compared with baseline levels. In contrast, the mRNA encoding TNF-α was significantly up-regulated in samples collected during both acute and late (>28. days post infection) phases of infection. There were also significantly higher levels of TNF-α mRNA expressed in samples derived from animals that were identified subsequently as persistently infected FMDV-carriers. It was concluded that there was a significant difference in the host-response in the DSP of calves that were identified as persistently infected, subclinical carriers of FMDV.

KW - Cytokine mRNA

KW - Foot-and-mouth disease

KW - Pharyngeal epithelium

UR - http://www.scopus.com/inward/record.url?scp=84860392113&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84860392113&partnerID=8YFLogxK

U2 - 10.1016/j.jcpa.2011.06.005

DO - 10.1016/j.jcpa.2011.06.005

M3 - Article

C2 - 21868026

AN - SCOPUS:84860392113

SN - 0021-9975

VL - 146

SP - 243

EP - 252

JO - Journal of Comparative Pathology

JF - Journal of Comparative Pathology

IS - 2-3

ER -

Modulation of Cytokine mRNA Expression in Pharyngeal Epithelial Samples obtained from Cattle Infected with Foot-and-Mouth Disease Virus

Abstract

Bibliographical note

Keywords

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this