Modulation of the oxidative stress and inflammatory cytokine response by thymoquinone in the collagen induced arthritis in Wistar rats

Sadiq Umar, Jamil Zargan, Khalid Umar, Sayeed Ahmad, Chandra Kant Katiyar, Haider A. Khan

Research output: Contribution to journalArticlepeer-review

117 Scopus citations

Abstract

Thymoquinone (TQ) is the major active compound derived from Nigella sativa. Our aim of this work was to evaluate the antioxidant and antiarthritic activity of TQ in Wistar rat by collagen induced arthritis (CIA). TQ was administered at a dose of 5 mg kg -1 body weight once daily for 21 days. The effects of treatment in the rats were assessed by biochemical (articular elastase, MPO, LPO, GSH, catalase, SOD and NO), inflammatory mediators (IL-1β, IL-6, TNF-α, IL-10, IFN-γ and PGE 2) and histological studies in joints. TQ was effective in bringing significant changes on all the parameters (articular elastase, MPO, LPO, GSH, catalase, SOD and NO) studied. Oral administration of TQ resulted in significantly reduced the levels of pro-inflammatory mediators (IL-1β, IL-6, TNF-α, IFN-γ and PGE 2) and increased level of IL-10. The protective effects of TQ against RA were also evident from the decrease in arthritis scoring and bone histology. In conclusion, the fact that TQ abolished a number of factors known to be involved in RA pathogenesis indicates that the administration of thymoquinone may have potential value in the treatment of inflammatory disease.

Original languageEnglish (US)
Pages (from-to)40-46
Number of pages7
JournalChemico-Biological Interactions
Volume197
Issue number1
DOIs
StatePublished - Apr 15 2012

Bibliographical note

Funding Information:
Author is grateful to Indian council of medical research, Government of India for providing the financial support in the form of senior research fellowship (SRF).

Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.

Keywords

  • Collagen induced arthritis
  • IL-6
  • Nitric oxide
  • PGE
  • TNF-α
  • Thymoquinone

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