TY - JOUR
T1 - Molecular cloning and characterization of a cDNA encoding a novel cuticle protein in the silkworm, Bombyx mori
AU - Sawada, Hiroshi
AU - Nakato, Hiroshi
AU - Togawa, Toru
AU - Nakagoshi, Motoko
AU - Takikawa, Shin Ichiro
AU - Dohke, Kenjiro
AU - Iino, Teruhiko
AU - Mase, Keisuke
AU - Yamamoto, Toshio
AU - Izumi, Susumu
PY - 2003/3/1
Y1 - 2003/3/1
N2 - We have cloned the full length of a novel cDNA named Bombyx mori cuticle protein that contains an AlaAlaProAla/Val-repeat (BMCPA) from a cDNA library of integument in the larval silkworm. Both a typical tandem repeat (A-A-P-A/V) for cuticle protein and a unique tandem repeat with Ser, Ala, Gly, Pro, Val, Tyr and Thr were observed in the predicted amino acid sequence of the cDNA encoding BMCPA. Approximately 80% of the amino acids in BMCPA were composed of Ser, Ala, Gly, Pro, Val and Tyr. Northern-hybridization analysis indicated that BMCPA mRNA is expressed only in the larval epidermis and that the expression pattern of the BMCPA gene in the developmental stage was observed mainly at the larval stage. We propose BMCPA may be a novel component of cuticle, and may play an important role in the integument of the larval silkworm.
AB - We have cloned the full length of a novel cDNA named Bombyx mori cuticle protein that contains an AlaAlaProAla/Val-repeat (BMCPA) from a cDNA library of integument in the larval silkworm. Both a typical tandem repeat (A-A-P-A/V) for cuticle protein and a unique tandem repeat with Ser, Ala, Gly, Pro, Val, Tyr and Thr were observed in the predicted amino acid sequence of the cDNA encoding BMCPA. Approximately 80% of the amino acids in BMCPA were composed of Ser, Ala, Gly, Pro, Val and Tyr. Northern-hybridization analysis indicated that BMCPA mRNA is expressed only in the larval epidermis and that the expression pattern of the BMCPA gene in the developmental stage was observed mainly at the larval stage. We propose BMCPA may be a novel component of cuticle, and may play an important role in the integument of the larval silkworm.
KW - Alanine-alanine-proline/valine-repeat
KW - Bombyx mori
KW - Cuticle protein
KW - Gene expression
KW - Primary structure
KW - R&R consensus
KW - RNA polymerase II
KW - cDNA cloning
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U2 - 10.1016/S1096-4959(03)00002-2
DO - 10.1016/S1096-4959(03)00002-2
M3 - Article
C2 - 12628383
AN - SCOPUS:0037333255
VL - 134
SP - 519
EP - 527
JO - Comparative Biochemistry and Physiology -- Part B: Biochemistry and
JF - Comparative Biochemistry and Physiology -- Part B: Biochemistry and
SN - 0305-0491
IS - 3
ER -