Molecular cloning and characterization of a cDNA encoding a novel cuticle protein in the silkworm, Bombyx mori

Hiroshi Sawada; Hiroshi Nakato; Toru Togawa; Motoko Nakagoshi; Shin Ichiro Takikawa; Kenjiro Dohke; Teruhiko Iino; Keisuke Mase; Toshio Yamamoto; Susumu Izumi

doi:10.1016/S1096-4959(03)00002-2

Molecular cloning and characterization of a cDNA encoding a novel cuticle protein in the silkworm, Bombyx mori

Hiroshi Sawada, Hiroshi Nakato, Toru Togawa, Motoko Nakagoshi, Shin Ichiro Takikawa, Kenjiro Dohke, Teruhiko Iino, Keisuke Mase, Toshio Yamamoto, Susumu Izumi

Genetics, Cell Biology and Development (CBS)

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

We have cloned the full length of a novel cDNA named Bombyx mori cuticle protein that contains an AlaAlaProAla/Val-repeat (BMCPA) from a cDNA library of integument in the larval silkworm. Both a typical tandem repeat (A-A-P-A/V) for cuticle protein and a unique tandem repeat with Ser, Ala, Gly, Pro, Val, Tyr and Thr were observed in the predicted amino acid sequence of the cDNA encoding BMCPA. Approximately 80% of the amino acids in BMCPA were composed of Ser, Ala, Gly, Pro, Val and Tyr. Northern-hybridization analysis indicated that BMCPA mRNA is expressed only in the larval epidermis and that the expression pattern of the BMCPA gene in the developmental stage was observed mainly at the larval stage. We propose BMCPA may be a novel component of cuticle, and may play an important role in the integument of the larval silkworm.

Original language	English (US)
Pages (from-to)	519-527
Number of pages	9
Journal	Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume	134
Issue number	3
DOIs	https://doi.org/10.1016/S1096-4959(03)00002-2
State	Published - Mar 1 2003

Bibliographical note

Funding Information:
This work was partially supported by Grant-in-Aid from Ministry of Education, Science and Culture of Japan (11760040 and 13760044) and Kitasato University Research Grant for Young Researchers.

Keywords

Alanine-alanine-proline/valine-repeat
Bombyx mori
Cuticle protein
Gene expression
Primary structure
R&R consensus
RNA polymerase II
cDNA cloning

Access

10.1016/S1096-4959(03)00002-2

OpenUrl availability

Full text

Cite this

Sawada, H., Nakato, H., Togawa, T., Nakagoshi, M., Takikawa, S. I., Dohke, K., Iino, T., Mase, K., Yamamoto, T., & Izumi, S. (2003). Molecular cloning and characterization of a cDNA encoding a novel cuticle protein in the silkworm, Bombyx mori. Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 134(3), 519-527. https://doi.org/10.1016/S1096-4959(03)00002-2

Sawada, H, Nakato, H, Togawa, T, Nakagoshi, M, Takikawa, SI, Dohke, K, Iino, T, Mase, K, Yamamoto, T & Izumi, S 2003, 'Molecular cloning and characterization of a cDNA encoding a novel cuticle protein in the silkworm, Bombyx mori', Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, vol. 134, no. 3, pp. 519-527. https://doi.org/10.1016/S1096-4959(03)00002-2

@article{594c8412825a4b628e8ca7ac75a38cc4,

title = "Molecular cloning and characterization of a cDNA encoding a novel cuticle protein in the silkworm, Bombyx mori",

abstract = "We have cloned the full length of a novel cDNA named Bombyx mori cuticle protein that contains an AlaAlaProAla/Val-repeat (BMCPA) from a cDNA library of integument in the larval silkworm. Both a typical tandem repeat (A-A-P-A/V) for cuticle protein and a unique tandem repeat with Ser, Ala, Gly, Pro, Val, Tyr and Thr were observed in the predicted amino acid sequence of the cDNA encoding BMCPA. Approximately 80% of the amino acids in BMCPA were composed of Ser, Ala, Gly, Pro, Val and Tyr. Northern-hybridization analysis indicated that BMCPA mRNA is expressed only in the larval epidermis and that the expression pattern of the BMCPA gene in the developmental stage was observed mainly at the larval stage. We propose BMCPA may be a novel component of cuticle, and may play an important role in the integument of the larval silkworm.",

keywords = "Alanine-alanine-proline/valine-repeat, Bombyx mori, Cuticle protein, Gene expression, Primary structure, R&R consensus, RNA polymerase II, cDNA cloning",

author = "Hiroshi Sawada and Hiroshi Nakato and Toru Togawa and Motoko Nakagoshi and Takikawa, {Shin Ichiro} and Kenjiro Dohke and Teruhiko Iino and Keisuke Mase and Toshio Yamamoto and Susumu Izumi",

note = "Funding Information: This work was partially supported by Grant-in-Aid from Ministry of Education, Science and Culture of Japan (11760040 and 13760044) and Kitasato University Research Grant for Young Researchers.",

year = "2003",

month = mar,

day = "1",

doi = "10.1016/S1096-4959(03)00002-2",

language = "English (US)",

volume = "134",

pages = "519--527",

journal = "Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology",

issn = "1096-4959",

publisher = "Elsevier Inc.",

number = "3",

}

TY - JOUR

T1 - Molecular cloning and characterization of a cDNA encoding a novel cuticle protein in the silkworm, Bombyx mori

AU - Sawada, Hiroshi

AU - Nakato, Hiroshi

AU - Togawa, Toru

AU - Nakagoshi, Motoko

AU - Takikawa, Shin Ichiro

AU - Dohke, Kenjiro

AU - Iino, Teruhiko

AU - Mase, Keisuke

AU - Yamamoto, Toshio

AU - Izumi, Susumu

N1 - Funding Information: This work was partially supported by Grant-in-Aid from Ministry of Education, Science and Culture of Japan (11760040 and 13760044) and Kitasato University Research Grant for Young Researchers.

PY - 2003/3/1

Y1 - 2003/3/1

N2 - We have cloned the full length of a novel cDNA named Bombyx mori cuticle protein that contains an AlaAlaProAla/Val-repeat (BMCPA) from a cDNA library of integument in the larval silkworm. Both a typical tandem repeat (A-A-P-A/V) for cuticle protein and a unique tandem repeat with Ser, Ala, Gly, Pro, Val, Tyr and Thr were observed in the predicted amino acid sequence of the cDNA encoding BMCPA. Approximately 80% of the amino acids in BMCPA were composed of Ser, Ala, Gly, Pro, Val and Tyr. Northern-hybridization analysis indicated that BMCPA mRNA is expressed only in the larval epidermis and that the expression pattern of the BMCPA gene in the developmental stage was observed mainly at the larval stage. We propose BMCPA may be a novel component of cuticle, and may play an important role in the integument of the larval silkworm.

AB - We have cloned the full length of a novel cDNA named Bombyx mori cuticle protein that contains an AlaAlaProAla/Val-repeat (BMCPA) from a cDNA library of integument in the larval silkworm. Both a typical tandem repeat (A-A-P-A/V) for cuticle protein and a unique tandem repeat with Ser, Ala, Gly, Pro, Val, Tyr and Thr were observed in the predicted amino acid sequence of the cDNA encoding BMCPA. Approximately 80% of the amino acids in BMCPA were composed of Ser, Ala, Gly, Pro, Val and Tyr. Northern-hybridization analysis indicated that BMCPA mRNA is expressed only in the larval epidermis and that the expression pattern of the BMCPA gene in the developmental stage was observed mainly at the larval stage. We propose BMCPA may be a novel component of cuticle, and may play an important role in the integument of the larval silkworm.

KW - Alanine-alanine-proline/valine-repeat

KW - Bombyx mori

KW - Cuticle protein

KW - Gene expression

KW - Primary structure

KW - R&R consensus

KW - RNA polymerase II

KW - cDNA cloning

UR - http://www.scopus.com/inward/record.url?scp=0037333255&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037333255&partnerID=8YFLogxK

U2 - 10.1016/S1096-4959(03)00002-2

DO - 10.1016/S1096-4959(03)00002-2

M3 - Article

C2 - 12628383

AN - SCOPUS:0037333255

SN - 1096-4959

VL - 134

SP - 519

EP - 527

JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

IS - 3

ER -

Molecular cloning and characterization of a cDNA encoding a novel cuticle protein in the silkworm, Bombyx mori

Abstract

Bibliographical note

Keywords

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this