We have cloned the full length of a novel cDNA named Bombyx mori cuticle protein that contains an AlaAlaProAla/Val-repeat (BMCPA) from a cDNA library of integument in the larval silkworm. Both a typical tandem repeat (A-A-P-A/V) for cuticle protein and a unique tandem repeat with Ser, Ala, Gly, Pro, Val, Tyr and Thr were observed in the predicted amino acid sequence of the cDNA encoding BMCPA. Approximately 80% of the amino acids in BMCPA were composed of Ser, Ala, Gly, Pro, Val and Tyr. Northern-hybridization analysis indicated that BMCPA mRNA is expressed only in the larval epidermis and that the expression pattern of the BMCPA gene in the developmental stage was observed mainly at the larval stage. We propose BMCPA may be a novel component of cuticle, and may play an important role in the integument of the larval silkworm.
|Original language||English (US)|
|Number of pages||9|
|Journal||Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology|
|State||Published - Mar 1 2003|
Bibliographical noteFunding Information:
This work was partially supported by Grant-in-Aid from Ministry of Education, Science and Culture of Japan (11760040 and 13760044) and Kitasato University Research Grant for Young Researchers.
- Bombyx mori
- Cuticle protein
- Gene expression
- Primary structure
- R&R consensus
- RNA polymerase II
- cDNA cloning