Molecular cloning and characterization of a cDNA encoding a novel cuticle protein in the silkworm, Bombyx mori

Hiroshi Sawada; Hiroshi Nakato; Toru Togawa; Motoko Nakagoshi; Shin Ichiro Takikawa; Kenjiro Dohke; Teruhiko Iino; Keisuke Mase; Toshio Yamamoto; Susumu Izumi

doi:10.1016/S1096-4959(03)00002-2

Molecular cloning and characterization of a cDNA encoding a novel cuticle protein in the silkworm, Bombyx mori

Hiroshi Sawada, Hiroshi Nakato, Toru Togawa, Motoko Nakagoshi, Shin Ichiro Takikawa, Kenjiro Dohke, Teruhiko Iino, Keisuke Mase, Toshio Yamamoto, Susumu Izumi

Genetics, Cell Biology and Development (CBS)

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

We have cloned the full length of a novel cDNA named Bombyx mori cuticle protein that contains an AlaAlaProAla/Val-repeat (BMCPA) from a cDNA library of integument in the larval silkworm. Both a typical tandem repeat (A-A-P-A/V) for cuticle protein and a unique tandem repeat with Ser, Ala, Gly, Pro, Val, Tyr and Thr were observed in the predicted amino acid sequence of the cDNA encoding BMCPA. Approximately 80% of the amino acids in BMCPA were composed of Ser, Ala, Gly, Pro, Val and Tyr. Northern-hybridization analysis indicated that BMCPA mRNA is expressed only in the larval epidermis and that the expression pattern of the BMCPA gene in the developmental stage was observed mainly at the larval stage. We propose BMCPA may be a novel component of cuticle, and may play an important role in the integument of the larval silkworm.

Original language	English (US)
Pages (from-to)	519-527
Number of pages	9
Journal	Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume	134
Issue number	3
DOIs	https://doi.org/10.1016/S1096-4959(03)00002-2
State	Published - Mar 1 2003

Keywords

Alanine-alanine-proline/valine-repeat
Bombyx mori
Cuticle protein
Gene expression
Primary structure
R&R consensus
RNA polymerase II
cDNA cloning

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Sawada, H., Nakato, H., Togawa, T., Nakagoshi, M., Takikawa, S. I., Dohke, K., Iino, T., Mase, K., Yamamoto, T., & Izumi, S. (2003). Molecular cloning and characterization of a cDNA encoding a novel cuticle protein in the silkworm, Bombyx mori. Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 134(3), 519-527. https://doi.org/10.1016/S1096-4959(03)00002-2

Molecular cloning and characterization of a cDNA encoding a novel cuticle protein in the silkworm, Bombyx mori. / Sawada, Hiroshi; Nakato, Hiroshi; Togawa, Toru; Nakagoshi, Motoko; Takikawa, Shin Ichiro; Dohke, Kenjiro; Iino, Teruhiko; Mase, Keisuke; Yamamoto, Toshio; Izumi, Susumu.

In: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, Vol. 134, No. 3, 01.03.2003, p. 519-527.

Research output: Contribution to journal › Article › peer-review

Sawada, H, Nakato, H, Togawa, T, Nakagoshi, M, Takikawa, SI, Dohke, K, Iino, T, Mase, K, Yamamoto, T & Izumi, S 2003, 'Molecular cloning and characterization of a cDNA encoding a novel cuticle protein in the silkworm, Bombyx mori', Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, vol. 134, no. 3, pp. 519-527. https://doi.org/10.1016/S1096-4959(03)00002-2

Sawada, Hiroshi ; Nakato, Hiroshi ; Togawa, Toru ; Nakagoshi, Motoko ; Takikawa, Shin Ichiro ; Dohke, Kenjiro ; Iino, Teruhiko ; Mase, Keisuke ; Yamamoto, Toshio ; Izumi, Susumu. / Molecular cloning and characterization of a cDNA encoding a novel cuticle protein in the silkworm, Bombyx mori. In: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology. 2003 ; Vol. 134, No. 3. pp. 519-527.

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abstract = "We have cloned the full length of a novel cDNA named Bombyx mori cuticle protein that contains an AlaAlaProAla/Val-repeat (BMCPA) from a cDNA library of integument in the larval silkworm. Both a typical tandem repeat (A-A-P-A/V) for cuticle protein and a unique tandem repeat with Ser, Ala, Gly, Pro, Val, Tyr and Thr were observed in the predicted amino acid sequence of the cDNA encoding BMCPA. Approximately 80% of the amino acids in BMCPA were composed of Ser, Ala, Gly, Pro, Val and Tyr. Northern-hybridization analysis indicated that BMCPA mRNA is expressed only in the larval epidermis and that the expression pattern of the BMCPA gene in the developmental stage was observed mainly at the larval stage. We propose BMCPA may be a novel component of cuticle, and may play an important role in the integument of the larval silkworm.",

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AU - Nakagoshi, Motoko

AU - Takikawa, Shin Ichiro

AU - Dohke, Kenjiro

AU - Iino, Teruhiko

AU - Mase, Keisuke

AU - Yamamoto, Toshio

AU - Izumi, Susumu

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N2 - We have cloned the full length of a novel cDNA named Bombyx mori cuticle protein that contains an AlaAlaProAla/Val-repeat (BMCPA) from a cDNA library of integument in the larval silkworm. Both a typical tandem repeat (A-A-P-A/V) for cuticle protein and a unique tandem repeat with Ser, Ala, Gly, Pro, Val, Tyr and Thr were observed in the predicted amino acid sequence of the cDNA encoding BMCPA. Approximately 80% of the amino acids in BMCPA were composed of Ser, Ala, Gly, Pro, Val and Tyr. Northern-hybridization analysis indicated that BMCPA mRNA is expressed only in the larval epidermis and that the expression pattern of the BMCPA gene in the developmental stage was observed mainly at the larval stage. We propose BMCPA may be a novel component of cuticle, and may play an important role in the integument of the larval silkworm.

AB - We have cloned the full length of a novel cDNA named Bombyx mori cuticle protein that contains an AlaAlaProAla/Val-repeat (BMCPA) from a cDNA library of integument in the larval silkworm. Both a typical tandem repeat (A-A-P-A/V) for cuticle protein and a unique tandem repeat with Ser, Ala, Gly, Pro, Val, Tyr and Thr were observed in the predicted amino acid sequence of the cDNA encoding BMCPA. Approximately 80% of the amino acids in BMCPA were composed of Ser, Ala, Gly, Pro, Val and Tyr. Northern-hybridization analysis indicated that BMCPA mRNA is expressed only in the larval epidermis and that the expression pattern of the BMCPA gene in the developmental stage was observed mainly at the larval stage. We propose BMCPA may be a novel component of cuticle, and may play an important role in the integument of the larval silkworm.

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KW - cDNA cloning

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