Molecular cloning and expression analysis of the Turkey vasoactive intestinal peptide receptor

Y. Seungkwon; C. C. Hsu; H. Kim; Y. Kho; Y. J. Choi; M. E. El Halawani; J. Farris; D. N. Foster

doi:10.1006/gcen.2001.7685

Molecular cloning and expression analysis of the Turkey vasoactive intestinal peptide receptor

Y. Seungkwon, C. C. Hsu, H. Kim, Y. Kho, Y. J. Choi, M. E. El Halawani, J. Farris, D. N. Foster

Animal Science

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Vasoactive intestinal peptide (VIP) is a prolactin (PRL)-releasing factor whose activity in avian species is believed to be mediated by a specific VIP receptor (VIP-R). Circulating PRL levels are closely related to hypothalamic VIP immunoreactivity, hypothalamic VIP mRNA content, and hypophysial-portal blood VIP concentrations in turkeys. In the present study, a turkey VIP-R (tVIP-R) cDNA was cloned and its mRNA abundance was quantified in various tissues during different reproductive stages. The 2347-bp tVIP-R cDNA encoded a 457 amino acid protein, with a predicted Mr of 52 kDa. The full-length cDNA shares approximately 55% similarity with the mammalian VIP receptor-1. Northern blot analysis revealed that a major 2.7-kb transcript was expressed in laying hen pituitaries. Furthermore, two minor tVIP-R transcripts of 3.7 and 3.4 kb were observed. Semiquantitative reverse transcription polymerase chain reaction (RT-PCR) was performed using RNA from various turkey brain and peripheral tissues throughout the reproductive cycle. The steady-state levels of pituitary tVIP-R mRNA changed during the reproductive cycle, whereas mRNA expression in other tissues was not affected. The steady-state levels of tVIP-R mRNA were only affected in the pituitary, whereas mRNA expression in any of the other tissues examined following the immunization of turkeys against VIP were not affected.

Original language	English (US)
Pages (from-to)	53-65
Number of pages	13
Journal	General and Comparative Endocrinology
Volume	124
Issue number	1
DOIs	https://doi.org/10.1006/gcen.2001.7685
State	Published - 2001

PubMed: MeSH publication types

Journal Article

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access

10.1006/gcen.2001.7685

OpenUrl availability

Full text

Cite this

@article{7f2a857c050c48b59f2c6b648b3842a5,

title = "Molecular cloning and expression analysis of the Turkey vasoactive intestinal peptide receptor",

abstract = "Vasoactive intestinal peptide (VIP) is a prolactin (PRL)-releasing factor whose activity in avian species is believed to be mediated by a specific VIP receptor (VIP-R). Circulating PRL levels are closely related to hypothalamic VIP immunoreactivity, hypothalamic VIP mRNA content, and hypophysial-portal blood VIP concentrations in turkeys. In the present study, a turkey VIP-R (tVIP-R) cDNA was cloned and its mRNA abundance was quantified in various tissues during different reproductive stages. The 2347-bp tVIP-R cDNA encoded a 457 amino acid protein, with a predicted Mr of 52 kDa. The full-length cDNA shares approximately 55% similarity with the mammalian VIP receptor-1. Northern blot analysis revealed that a major 2.7-kb transcript was expressed in laying hen pituitaries. Furthermore, two minor tVIP-R transcripts of 3.7 and 3.4 kb were observed. Semiquantitative reverse transcription polymerase chain reaction (RT-PCR) was performed using RNA from various turkey brain and peripheral tissues throughout the reproductive cycle. The steady-state levels of pituitary tVIP-R mRNA changed during the reproductive cycle, whereas mRNA expression in other tissues was not affected. The steady-state levels of tVIP-R mRNA were only affected in the pituitary, whereas mRNA expression in any of the other tissues examined following the immunization of turkeys against VIP were not affected.",

author = "Y. Seungkwon and Hsu, {C. C.} and H. Kim and Y. Kho and Choi, {Y. J.} and {El Halawani}, {M. E.} and J. Farris and Foster, {D. N.}",

year = "2001",

doi = "10.1006/gcen.2001.7685",

language = "English (US)",

volume = "124",

pages = "53--65",

journal = "General and Comparative Endocrinology",

issn = "0016-6480",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Molecular cloning and expression analysis of the Turkey vasoactive intestinal peptide receptor

AU - Seungkwon, Y.

AU - Hsu, C. C.

AU - Kim, H.

AU - Kho, Y.

AU - Choi, Y. J.

AU - El Halawani, M. E.

AU - Farris, J.

AU - Foster, D. N.

PY - 2001

Y1 - 2001

N2 - Vasoactive intestinal peptide (VIP) is a prolactin (PRL)-releasing factor whose activity in avian species is believed to be mediated by a specific VIP receptor (VIP-R). Circulating PRL levels are closely related to hypothalamic VIP immunoreactivity, hypothalamic VIP mRNA content, and hypophysial-portal blood VIP concentrations in turkeys. In the present study, a turkey VIP-R (tVIP-R) cDNA was cloned and its mRNA abundance was quantified in various tissues during different reproductive stages. The 2347-bp tVIP-R cDNA encoded a 457 amino acid protein, with a predicted Mr of 52 kDa. The full-length cDNA shares approximately 55% similarity with the mammalian VIP receptor-1. Northern blot analysis revealed that a major 2.7-kb transcript was expressed in laying hen pituitaries. Furthermore, two minor tVIP-R transcripts of 3.7 and 3.4 kb were observed. Semiquantitative reverse transcription polymerase chain reaction (RT-PCR) was performed using RNA from various turkey brain and peripheral tissues throughout the reproductive cycle. The steady-state levels of pituitary tVIP-R mRNA changed during the reproductive cycle, whereas mRNA expression in other tissues was not affected. The steady-state levels of tVIP-R mRNA were only affected in the pituitary, whereas mRNA expression in any of the other tissues examined following the immunization of turkeys against VIP were not affected.

AB - Vasoactive intestinal peptide (VIP) is a prolactin (PRL)-releasing factor whose activity in avian species is believed to be mediated by a specific VIP receptor (VIP-R). Circulating PRL levels are closely related to hypothalamic VIP immunoreactivity, hypothalamic VIP mRNA content, and hypophysial-portal blood VIP concentrations in turkeys. In the present study, a turkey VIP-R (tVIP-R) cDNA was cloned and its mRNA abundance was quantified in various tissues during different reproductive stages. The 2347-bp tVIP-R cDNA encoded a 457 amino acid protein, with a predicted Mr of 52 kDa. The full-length cDNA shares approximately 55% similarity with the mammalian VIP receptor-1. Northern blot analysis revealed that a major 2.7-kb transcript was expressed in laying hen pituitaries. Furthermore, two minor tVIP-R transcripts of 3.7 and 3.4 kb were observed. Semiquantitative reverse transcription polymerase chain reaction (RT-PCR) was performed using RNA from various turkey brain and peripheral tissues throughout the reproductive cycle. The steady-state levels of pituitary tVIP-R mRNA changed during the reproductive cycle, whereas mRNA expression in other tissues was not affected. The steady-state levels of tVIP-R mRNA were only affected in the pituitary, whereas mRNA expression in any of the other tissues examined following the immunization of turkeys against VIP were not affected.

UR - http://www.scopus.com/inward/record.url?scp=0034763340&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034763340&partnerID=8YFLogxK

U2 - 10.1006/gcen.2001.7685

DO - 10.1006/gcen.2001.7685

M3 - Article

C2 - 11703071

AN - SCOPUS:0034763340

SN - 0016-6480

VL - 124

SP - 53

EP - 65

JO - General and Comparative Endocrinology

JF - General and Comparative Endocrinology

IS - 1

ER -

Molecular cloning and expression analysis of the Turkey vasoactive intestinal peptide receptor

Abstract

PubMed: MeSH publication types

UN SDGs

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this