Negative regulation of adiponectin receptor 1 promoter by insulin via a repressive nuclear inhibitory protein element

Xiaolan Sun; Jing He; Chenqian Mao; Ruijun Han; Zhenzhen Wang; Yong Liu; Yan Chen

doi:10.1016/j.febslet.2008.08.037

Negative regulation of adiponectin receptor 1 promoter by insulin via a repressive nuclear inhibitory protein element

Xiaolan Sun, Jing He, Chenqian Mao, Ruijun Han, Zhenzhen Wang, Yong Liu, Yan Chen

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Adiponectin is an adipose-derived hormone that has anti-diabetic and anti-atherogenic effects through interaction with adiponectin receptors AdipoR1 and AdipoR2. We analyzed the transcriptional regulation of AdipoR1 by insulin. Insulin repressed the promoter activity of AdipoR1 in C2C12 myoblasts via PI3K and Foxo1. Deletion studies demonstrated the presence of a putative insulin-responsive region which is composed of a nuclear inhibitory protein (NIP) binding element. Mutation of the NIP element abrogated the negative regulation of AdipoR1 promoter by insulin. Insulin treatment could induce formation of a protein complex that bound the NIP element. Collectively, our data suggest that a repressive NIP element is involved in the negative regulation of AdipoR1 promoter by insulin.

Original language	English (US)
Pages (from-to)	3401-3407
Number of pages	7
Journal	FEBS Letters
Volume	582
Issue number	23-24
DOIs	https://doi.org/10.1016/j.febslet.2008.08.037
State	Published - Oct 15 2008
Externally published	Yes

Bibliographical note

Funding Information:
We wish to thank Dr. K.L. Guan and Dr. Eric D. Tang from Institute of Gerontology, University of Michigan Medical School Ann Arbor, USA, for providing the plasmids. This work was supported by research grants from Chinese Academy of Sciences (One Hundred Talents Program and the Knowledge Innovation Program KSCX1-YW-02), National Natural Science Foundation of China (30588002 and 30470870), and the Ministry of Science and Technology of China (2007CB947100 and 2007CB947100) to Y.C.

Keywords

Adiponectin receptor
Foxo1
Insulin
Nuclear inhibitory protein element

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Negative regulation of adiponectin receptor 1 promoter by insulin via a repressive nuclear inhibitory protein element",

abstract = "Adiponectin is an adipose-derived hormone that has anti-diabetic and anti-atherogenic effects through interaction with adiponectin receptors AdipoR1 and AdipoR2. We analyzed the transcriptional regulation of AdipoR1 by insulin. Insulin repressed the promoter activity of AdipoR1 in C2C12 myoblasts via PI3K and Foxo1. Deletion studies demonstrated the presence of a putative insulin-responsive region which is composed of a nuclear inhibitory protein (NIP) binding element. Mutation of the NIP element abrogated the negative regulation of AdipoR1 promoter by insulin. Insulin treatment could induce formation of a protein complex that bound the NIP element. Collectively, our data suggest that a repressive NIP element is involved in the negative regulation of AdipoR1 promoter by insulin.",

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note = "Funding Information: We wish to thank Dr. K.L. Guan and Dr. Eric D. Tang from Institute of Gerontology, University of Michigan Medical School Ann Arbor, USA, for providing the plasmids. This work was supported by research grants from Chinese Academy of Sciences (One Hundred Talents Program and the Knowledge Innovation Program KSCX1-YW-02), National Natural Science Foundation of China (30588002 and 30470870), and the Ministry of Science and Technology of China (2007CB947100 and 2007CB947100) to Y.C.",

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AU - Sun, Xiaolan

AU - He, Jing

AU - Mao, Chenqian

AU - Han, Ruijun

AU - Wang, Zhenzhen

AU - Liu, Yong

AU - Chen, Yan

N1 - Funding Information: We wish to thank Dr. K.L. Guan and Dr. Eric D. Tang from Institute of Gerontology, University of Michigan Medical School Ann Arbor, USA, for providing the plasmids. This work was supported by research grants from Chinese Academy of Sciences (One Hundred Talents Program and the Knowledge Innovation Program KSCX1-YW-02), National Natural Science Foundation of China (30588002 and 30470870), and the Ministry of Science and Technology of China (2007CB947100 and 2007CB947100) to Y.C.

PY - 2008/10/15

Y1 - 2008/10/15

N2 - Adiponectin is an adipose-derived hormone that has anti-diabetic and anti-atherogenic effects through interaction with adiponectin receptors AdipoR1 and AdipoR2. We analyzed the transcriptional regulation of AdipoR1 by insulin. Insulin repressed the promoter activity of AdipoR1 in C2C12 myoblasts via PI3K and Foxo1. Deletion studies demonstrated the presence of a putative insulin-responsive region which is composed of a nuclear inhibitory protein (NIP) binding element. Mutation of the NIP element abrogated the negative regulation of AdipoR1 promoter by insulin. Insulin treatment could induce formation of a protein complex that bound the NIP element. Collectively, our data suggest that a repressive NIP element is involved in the negative regulation of AdipoR1 promoter by insulin.

AB - Adiponectin is an adipose-derived hormone that has anti-diabetic and anti-atherogenic effects through interaction with adiponectin receptors AdipoR1 and AdipoR2. We analyzed the transcriptional regulation of AdipoR1 by insulin. Insulin repressed the promoter activity of AdipoR1 in C2C12 myoblasts via PI3K and Foxo1. Deletion studies demonstrated the presence of a putative insulin-responsive region which is composed of a nuclear inhibitory protein (NIP) binding element. Mutation of the NIP element abrogated the negative regulation of AdipoR1 promoter by insulin. Insulin treatment could induce formation of a protein complex that bound the NIP element. Collectively, our data suggest that a repressive NIP element is involved in the negative regulation of AdipoR1 promoter by insulin.

KW - Adiponectin receptor

KW - Foxo1

KW - Insulin

KW - Nuclear inhibitory protein element

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Negative regulation of adiponectin receptor 1 promoter by insulin via a repressive nuclear inhibitory protein element

Abstract

Bibliographical note

Keywords

UN SDGs

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