Neutrophils promote VLA-4–dependent B cell antigen presentation and accumulation within the meninges during neuroinflammation

Chelsea R. Parker Harp, Angela S. Archambault, Matthew Cheung, Jesse W. Williams, Rafael S. Czepielewski, Patrick C. Duncker, Aaron J. Kilgore, Aidan T. Miller, Benjamin M. Segal, Alfred H.J. Kim, Gwendalyn J. Randolph, Gregory F. Wu

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The success of B cell depletion therapies and identification of leptomeningeal ectopic lymphoid tissue (ELT) in patients with multiple sclerosis (MS) has renewed interest in the antibody-independent pathogenic functions of B cells during neuroinflammation. The timing and location of B cell antigen presentation during MS and its animal model experimental autoimmune encephalomyelitis (EAE) remain undefined. Using a new EAE system that incorporates temporal regulation of MHCII expression by myelin-specific B cells, we observed the rapid formation of large B cell clusters in the spinal cord subarachnoid space. Neutrophils preceded the accumulation of meningeal B cell clusters, and inhibition of CXCR2-mediated granulocyte trafficking to the central nervous system reduced pathogenic B cell clusters and disease severity. Further, B cell-restricted very late antigen-4 (VLA-4) deficiency abrogated EAE dependent on B cell antigen presentation. Together, our findings demonstrate that neutrophils coordinate VLA-4–dependent B cell accumulation within the meninges during neuroinflammation, a key early step in the formation of ELT observed in MS.

Original languageEnglish (US)
Pages (from-to)24221-24230
Number of pages10
JournalProceedings of the National Academy of Sciences of the United States of America
Volume116
Issue number48
DOIs
StatePublished - Nov 26 2019

Bibliographical note

Funding Information:
ACKNOWLEDGMENTS. We thank Denise Dorsey, Christine Pham, Mary Dinauer, and Julia Sim for research advice and Thalia Papayannopoulou at the University of Washington for kindly providing itga4f/f mice. We would also like to thank Diane Bender and Michael Shih at Washington University for technical assistance. The National Institute of Neurological Disorders and Stroke supported C.R.P.H. (F31NS096824) and G.F.W. (R01NS106289). Research reported herein was supported by the Immunomonitoring Laboratory within The Andrew M. and Jane M. Bursky Center for Human Immunology and Immunotherapy Programs at Washington University in St. Louis.

Keywords

  • B cell
  • EAE
  • Multiple sclerosis

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