Abstract
The ubiquitous signaling molecule nitric oxide (NO) plays an important role in seed biology. Experiments with this biologically important gas require special provisions because NO in aerobic environments is readily converted into other oxides of nitrogen. In this chapter, we describe methods for the application of NO as a gas, and through the use of NO-donor compounds. We included information on the removal or reduction of NO with NO scavengers. Methods for detecting NO using NO-reactive fluorescent probes, and an apparatus incorporating an oxidizer column are also described.
| Original language | English (US) |
|---|---|
| Title of host publication | Seed Dormancy |
| Subtitle of host publication | Methods and Protocols |
| Publisher | Humana Press Inc. |
| Pages | 385-400 |
| Number of pages | 16 |
| ISBN (Print) | 9781617792304 |
| DOIs | |
| State | Published - 2011 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 773 |
| ISSN (Print) | 1064-3745 |
| ISSN (Electronic) | 1940-6029 |
Bibliographical note
Publisher Copyright:© 2011, Springer Science+Business Media, LLC.
Keywords
- Application of nitric oxide
- Nitrate and nitrite
- Nitric oxide detection
- Nitric oxide donors and scavengers
- Oxidation of nitric oxide
- Seed dormancy and germination