Non-invasive bat species identification from mixed-species samples using a microarray

Lorelei E. Patrick; Jan M. Just; Maarten J. Vonhof

doi:10.1007/s12686-016-0613-0

Non-invasive bat species identification from mixed-species samples using a microarray

Lorelei E. Patrick, Jan M. Just, Maarten J. Vonhof

Biology Teaching and Learning

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3 Scopus citations

Abstract

Identifying species from non-invasively collected, mixed species samples for biodiversity monitoring can be difficult and expensive using typical molecular methods because samples are often degraded. This is the case when identifying the species of bats present in roosts where guano may be the only means of assessment without disturbing the bats themselves. To aid in such studies, we developed species-specific DNA probes and a microarray capable of identifying most bat species in the United States and Canada using our existing database of 16S mitochondrial DNA sequences. The microarray was sensitive enough to detect DNA diluted 1:500 for several species combinations and was able to detect the presence of more species in mixed guano samples collected from roosts than did direct sequencing from individual fecal pellets. We suggest that these DNA probes and the microarray could be a valuable tool with which to non-invasively monitor bat populations and roost use.

Original language	English (US)
Pages (from-to)	139-149
Number of pages	11
Journal	Conservation Genetics Resources
Volume	9
Issue number	1
DOIs	https://doi.org/10.1007/s12686-016-0613-0
State	Published - Mar 1 2017

Bibliographical note

Funding Information:
Funding for this study was provided by the U.S. Army Corps of Engineers—Engineer Research and Development Center (Grants W9132T-05-C-0011 and W9132T-06-C-0010 through Portland State University). Laboratory work was conducted at Portland State University, and we wish to thank Dr. Deborah Duffield for lab space during this project. Matt Holman served as point of contact for the U.S. Army Corps of Engineers and we thank him for his logistical support and sample collection. Ammon Wilhelm also provided mixed species guano samples used in validation tests. Patricia Ormsbee and Aimee Hart provided samples and read previous versions of this manuscript. Caprice Rosato at Oregon State University offered invaluable advice and expertise developing and troubleshooting the array design.

Publisher Copyright:
© 2016, Springer Science+Business Media Dordrecht.

Keywords

Bat
Degraded DNA
Guano
Microarray
Mixed species samples
Non-invasive sampling
Species identification

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Non-invasive bat species identification from mixed-species samples using a microarray

Abstract

Bibliographical note

Keywords

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