Liquid storage of hematopoietic stem and progenitor cells often is necessary in clinical cell engineering. The interval between cell harvest and transplantation or processing may be as long as 1 or 2 days. We previously developed an infusible-grade solution, STM-Sav, for nonfrozen storage of peripheral blood stem cells. This study addresses the validation of the storage conditions using STM-Sav for the short-term storage of bone marrow. Bone marrow from normal donors was stored in STM-Sav in gas-permeable Cryocyte and plasma transfer bags at 4°C and room temperature. Cell viability, number, and morphology, percentage of CD34+ cells, frequency of granulocyte/macrophage colony-forming units (CFU-GM), and solution pH were measured every 24 h for up to 72 h. More than 88% of viable nucleated cell recovery was observed at 72 h of liquid storage. CD34+ cell recoveries were unchanged over the 72-h storage period as well, and the frequency of CFU-GM per 105 cells was also constant. The regulation of solution pH was improved with storage of the cells at 4°C in a plasma transfer bag. These results indicate that STM-Sav can be effective in preserving bone marrow viability over a 72-h period during liquid storage.
|Original language||English (US)|
|Number of pages||7|
|Journal||Cell Preservation Technology|
|State||Published - Jul 21 2003|