Observation of a second phosphate pool in the perfused heart by 31P NMR; is this the mitochondrial phosphate?

Pamela B. Garlick; Truman R. Brown; Richard H. Sullivan; Kamil Ugurbil

doi:10.1016/0022-2828(83)90347-4

Observation of a second phosphate pool in the perfused heart by ³¹P NMR; is this the mitochondrial phosphate?

Pamela B. Garlick, Truman R. Brown, Richard H. Sullivan, Kamil Ugurbil

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34 Scopus citations

Abstract

We report here our observations on perfused rat hearts using ³¹P NMR which show that inorganic phosphate (P_i) is located in two regions of differing pH. From the chemical shifts of the P_i signals and their behaviour under various stimuli we identify one signal (pH 7.0) as coming from the cytosol and suggest that the other (pH 7.38) comes from the mitochondrial matrix space.

Original language	English (US)
Pages (from-to)	855-858
Number of pages	4
Journal	Journal of Molecular and Cellular Cardiology
Volume	15
Issue number	12
DOIs	https://doi.org/10.1016/0022-2828(83)90347-4
State	Published - Dec 1983
Externally published	Yes

Bibliographical note

Funding Information:
by NSF and NIH

Keywords

Mitochondrial pH
Valinomycin

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10.1016/0022-2828(83)90347-4

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Cite this

@article{a960a2ee55164ea88dd3787625c5b33b,

title = "Observation of a second phosphate pool in the perfused heart by 31P NMR; is this the mitochondrial phosphate?",

abstract = "We report here our observations on perfused rat hearts using 31P NMR which show that inorganic phosphate (Pi) is located in two regions of differing pH. From the chemical shifts of the Pi signals and their behaviour under various stimuli we identify one signal (pH 7.0) as coming from the cytosol and suggest that the other (pH 7.38) comes from the mitochondrial matrix space.",

keywords = "Mitochondrial pH, Valinomycin",

author = "Garlick, {Pamela B.} and Brown, {Truman R.} and Sullivan, {Richard H.} and Kamil Ugurbil",

note = "Funding Information: by NSF and NIH",

year = "1983",

month = dec,

doi = "10.1016/0022-2828(83)90347-4",

language = "English (US)",

volume = "15",

pages = "855--858",

journal = "Journal of Molecular and Cellular Cardiology",

issn = "0022-2828",