Oct4+ stem/progenitor swine lung epithelial cells are targets for influenza virus replication

Mahesh Khatri, Sagar M. Goyal, Yehia M. Saif

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

We isolated stem/progenitor epithelial cells from the lungs of 4- to 6-week-old pigs. The epithelial progenitor colony cells were surrounded by mesenchymal stromal cells. The progenitor epithelial colony cells expressed stem cell markers such as octamer binding transcription factor 4 (Oct4) and stage-specific embryonic antigen 1 (SSEA-1), as well as the epithelial markers pancytokeratin, cytokeratin-18, and occludin, but not mesenchymal (CD44, CD29, and CD90) and hematopoietic (CD45) markers. The colony cells had extensive self-renewal potential and had the capacity to undergo differentiation to alveolar type I- and type II-like pneumocytes. Additionally, these cells expressed sialic acid receptors and supported the active replication of influenza virus, which was accompanied by cell lysis. The lysis of progenitor epithelial cells by influenza virus may cause a marked reduction in the potential of progenitor cells for self renewal and for their ability to differentiate into specialized cells of the lung. These observations suggest the possible involvement of lung stem/progenitor cells in influenza virus infection.

Original languageEnglish (US)
Pages (from-to)6427-6433
Number of pages7
JournalJournal of virology
Volume86
Issue number12
DOIs
StatePublished - Jul 2012

Bibliographical note

Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.

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