Abstract
A rat brain cDNA library was screened with probes constructed from portions of the cDNA (OBCAM) encoding the opioid-binding cell adhesion molecule (OBCAM). Three clones of interest were isolated and sequenced. The largest clone, DUZ1, had a putative open reading frame (ORF) essentially identical to OBCAM in its C-terminal 318 amino acids (aa), but differing in its N-terminal aa (20 vs. 27 in OBCAM), and in all of its 5′-noncoding regions. The other clones, SG8 and SG13, had a putative ORF essentially identical to that of OBCAM, but differed from each other in a portion of their 5′-noncoding region. This study suggests that there is a family of OBCAM-like genes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 249-254 |
| Number of pages | 6 |
| Journal | Gene |
| Volume | 117 |
| Issue number | 2 |
| DOIs | |
| State | Published - Aug 15 1992 |
Bibliographical note
Funding Information:This work was supported in part by NIDA research grants 00564 (H.H.L.), 05695 (H.H.L.), 01583 (H.H.L.), 02643 (N.M.L.), and Research Scientist Award K05-DA70554 (H.H.L.) and K05-DA00020 (N.M.L.) and the F. Stark endowed Fund in Minnesota Medical Foundation. D.A.L. is currently registered in the graduate program in the Department of Pharmacology, University of California, San Francisco.
Keywords
- Opioid receptor
- cDNA cloning
- cell adhesion molecules
- immunoglobulin superfamily