Optogenetic activation of cholinergic neurons in the PPT or LDT induces REM sleep

Christa J. Van Dort, Daniel P. Zachs, Jonathan D. Kenny, Shu Zheng, Rebecca R. Goldblum, Noah A. Gelwan, Daniel M. Ramos, Michael A. Nolan, Karen Wang, Feng Ju Weng, Yingxi Lin, Matthew A. Wilson, Emery N. Brown

Research output: Contribution to journalArticlepeer-review

129 Scopus citations

Abstract

Rapid eye movement (REM) sleep is an important component of the natural sleep/wake cycle, yet the mechanisms that regulate REM sleep remain incompletely understood. Cholinergic neurons in the mesopontine tegmentum have been implicated in REM sleep regulation, but lesions of this area have had varying effects on REM sleep. Therefore, this study aimed to clarify the role of cholinergic neurons in the pedunculopontine tegmentum (PPT) and laterodorsal tegmentum (LDT) in REM sleep generation. Selective optogenetic activation of cholinergic neurons in the PPT or LDT during non-REM (NREM) sleep increased the number of REM sleep episodes and did not change REM sleep episode duration. Activation of cholinergic neurons in the PPT or LDT during NREM sleep was sufficient to induce REM sleep.

Original languageEnglish (US)
Pages (from-to)584-589
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume112
Issue number2
DOIs
StatePublished - Jan 13 2015
Externally publishedYes

Keywords

  • Acetylcholine
  • Mesopontine tegmentum
  • Mouse
  • Optogenetics
  • Rapid eye movement sleep

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