OsJAR1 is required for JA-regulated floret opening and anther dehiscence in rice

Yuguo Xiao; Yi Chen; Tatsiana Charnikhova; Patrick P.J. Mulder; Jeroen Heijmans; Angela Hoogenboom; Adamantia Agalou; Corinne Michel; Jean Benoit Morel; Ludovico Dreni; Martin M. Kater; Harro Bouwmeester; Mei Wang; Zhen Zhu; Pieter B.F. Ouwerkerk

doi:10.1007/s11103-014-0212-y

OsJAR1 is required for JA-regulated floret opening and anther dehiscence in rice

Yuguo Xiao, Yi Chen, Tatsiana Charnikhova, Patrick P.J. Mulder, Jeroen Heijmans, Angela Hoogenboom, Adamantia Agalou, Corinne Michel, Jean Benoit Morel, Ludovico Dreni, Martin M. Kater, Harro Bouwmeester, Mei Wang, Zhen Zhu, Pieter B.F. Ouwerkerk

Plant and Microbial Biology

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Abstract

Jasmonates are important phytohormones regulating reproductive development. We used two recessive rice Tos17 alleles of OsJAR1, osjar1-2 and osjar1-3, to study the biological function of jasmonates in rice anthesis. The florets of both osjar1 alleles stayed open during anthesis because the lodicules, which control flower opening in rice, were not withering on time. Furthermore, dehiscence of the anthers filled with viable pollen, was impaired, resulting in lower fertility. In situ hybridization and promoter GUS transgenic analysis confirmed OsJAR1 expression in these floral tissues. Flower opening induced by exogenous applied methyl jasmonate was impaired in osjar1 plants and was restored in a complementation experiment with transgenics expressing a wild type copy of OsJAR1 controlled by a rice actin promoter. Biochemical analysis showed that OsJAR1 encoded an enzyme conjugating jasmonic acid (JA) to at least Ile, Leu, Met, Phe, Trp and Val and both osjar1 alleles had substantial reduction in content of JA-Ile, JA-Leu and JA-Val in florets. We conclude that OsJAR1 is a JA-amino acid synthetase that is required for optimal flower opening and closing and anther dehiscence in rice.

Original language	English (US)
Pages (from-to)	19-33
Number of pages	15
Journal	Plant molecular biology
Volume	86
Issue number	1-2
DOIs	https://doi.org/10.1007/s11103-014-0212-y
State	Published - Sep 2014

Bibliographical note

Funding Information:
Acknowledgments The Tos17 lines used in this work were developed by Drs. Akio Miyao and Hirohiko Hirochika (Miyao et al. 2003) and were provided by the Rice Genome Resource Center (Tsukuba, Japan). We are grateful to Gerda Lamers for her technical support in microscopy. We thank Ward de Winter for tissue culture media and Elly Schrijnemakers for plant caretaking. This research was supported by the Programme for Strategic Scientific Alliances (04-PSA-BD-04) between China and the Netherlands of the Royal Netherlands Academy of Arts and Sciences (KNAW) for MW, PBFO, YX, ZZ and the KNAW China Exchange Programme (KNAW-CEP 04CDP022, 06CDO033, 07CDP005, 08CDP042) for YX and YC. We acknowledge financial support from the Netherlands Organization for Scientific Research (NWO; VICI-grant) to HJB, TC and PPJM.

Keywords

Arabidopsis
JA
JA-Ile
MeJA
OsJAR1
Rice

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Xiao, Y., Chen, Y., Charnikhova, T., Mulder, P. P. J., Heijmans, J., Hoogenboom, A., Agalou, A., Michel, C., Morel, J. B., Dreni, L., Kater, M. M., Bouwmeester, H., Wang, M., Zhu, Z., & Ouwerkerk, P. B. F. (2014). OsJAR1 is required for JA-regulated floret opening and anther dehiscence in rice. Plant molecular biology, 86(1-2), 19-33. https://doi.org/10.1007/s11103-014-0212-y

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title = "OsJAR1 is required for JA-regulated floret opening and anther dehiscence in rice",

abstract = "Jasmonates are important phytohormones regulating reproductive development. We used two recessive rice Tos17 alleles of OsJAR1, osjar1-2 and osjar1-3, to study the biological function of jasmonates in rice anthesis. The florets of both osjar1 alleles stayed open during anthesis because the lodicules, which control flower opening in rice, were not withering on time. Furthermore, dehiscence of the anthers filled with viable pollen, was impaired, resulting in lower fertility. In situ hybridization and promoter GUS transgenic analysis confirmed OsJAR1 expression in these floral tissues. Flower opening induced by exogenous applied methyl jasmonate was impaired in osjar1 plants and was restored in a complementation experiment with transgenics expressing a wild type copy of OsJAR1 controlled by a rice actin promoter. Biochemical analysis showed that OsJAR1 encoded an enzyme conjugating jasmonic acid (JA) to at least Ile, Leu, Met, Phe, Trp and Val and both osjar1 alleles had substantial reduction in content of JA-Ile, JA-Leu and JA-Val in florets. We conclude that OsJAR1 is a JA-amino acid synthetase that is required for optimal flower opening and closing and anther dehiscence in rice.",

keywords = "Arabidopsis, JA, JA-Ile, MeJA, OsJAR1, Rice",

author = "Yuguo Xiao and Yi Chen and Tatsiana Charnikhova and Mulder, {Patrick P.J.} and Jeroen Heijmans and Angela Hoogenboom and Adamantia Agalou and Corinne Michel and Morel, {Jean Benoit} and Ludovico Dreni and Kater, {Martin M.} and Harro Bouwmeester and Mei Wang and Zhen Zhu and Ouwerkerk, {Pieter B.F.}",

note = "Funding Information: Acknowledgments The Tos17 lines used in this work were developed by Drs. Akio Miyao and Hirohiko Hirochika (Miyao et al. 2003) and were provided by the Rice Genome Resource Center (Tsukuba, Japan). We are grateful to Gerda Lamers for her technical support in microscopy. We thank Ward de Winter for tissue culture media and Elly Schrijnemakers for plant caretaking. This research was supported by the Programme for Strategic Scientific Alliances (04-PSA-BD-04) between China and the Netherlands of the Royal Netherlands Academy of Arts and Sciences (KNAW) for MW, PBFO, YX, ZZ and the KNAW China Exchange Programme (KNAW-CEP 04CDP022, 06CDO033, 07CDP005, 08CDP042) for YX and YC. We acknowledge financial support from the Netherlands Organization for Scientific Research (NWO; VICI-grant) to HJB, TC and PPJM.",

year = "2014",

month = sep,

doi = "10.1007/s11103-014-0212-y",

language = "English (US)",

volume = "86",

pages = "19--33",

journal = "Plant molecular biology",

issn = "0167-4412",

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number = "1-2",

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TY - JOUR

T1 - OsJAR1 is required for JA-regulated floret opening and anther dehiscence in rice

AU - Xiao, Yuguo

AU - Chen, Yi

AU - Charnikhova, Tatsiana

AU - Mulder, Patrick P.J.

AU - Heijmans, Jeroen

AU - Hoogenboom, Angela

AU - Agalou, Adamantia

AU - Michel, Corinne

AU - Morel, Jean Benoit

AU - Dreni, Ludovico

AU - Kater, Martin M.

AU - Bouwmeester, Harro

AU - Wang, Mei

AU - Zhu, Zhen

AU - Ouwerkerk, Pieter B.F.

N1 - Funding Information: Acknowledgments The Tos17 lines used in this work were developed by Drs. Akio Miyao and Hirohiko Hirochika (Miyao et al. 2003) and were provided by the Rice Genome Resource Center (Tsukuba, Japan). We are grateful to Gerda Lamers for her technical support in microscopy. We thank Ward de Winter for tissue culture media and Elly Schrijnemakers for plant caretaking. This research was supported by the Programme for Strategic Scientific Alliances (04-PSA-BD-04) between China and the Netherlands of the Royal Netherlands Academy of Arts and Sciences (KNAW) for MW, PBFO, YX, ZZ and the KNAW China Exchange Programme (KNAW-CEP 04CDP022, 06CDO033, 07CDP005, 08CDP042) for YX and YC. We acknowledge financial support from the Netherlands Organization for Scientific Research (NWO; VICI-grant) to HJB, TC and PPJM.

PY - 2014/9

Y1 - 2014/9

N2 - Jasmonates are important phytohormones regulating reproductive development. We used two recessive rice Tos17 alleles of OsJAR1, osjar1-2 and osjar1-3, to study the biological function of jasmonates in rice anthesis. The florets of both osjar1 alleles stayed open during anthesis because the lodicules, which control flower opening in rice, were not withering on time. Furthermore, dehiscence of the anthers filled with viable pollen, was impaired, resulting in lower fertility. In situ hybridization and promoter GUS transgenic analysis confirmed OsJAR1 expression in these floral tissues. Flower opening induced by exogenous applied methyl jasmonate was impaired in osjar1 plants and was restored in a complementation experiment with transgenics expressing a wild type copy of OsJAR1 controlled by a rice actin promoter. Biochemical analysis showed that OsJAR1 encoded an enzyme conjugating jasmonic acid (JA) to at least Ile, Leu, Met, Phe, Trp and Val and both osjar1 alleles had substantial reduction in content of JA-Ile, JA-Leu and JA-Val in florets. We conclude that OsJAR1 is a JA-amino acid synthetase that is required for optimal flower opening and closing and anther dehiscence in rice.

AB - Jasmonates are important phytohormones regulating reproductive development. We used two recessive rice Tos17 alleles of OsJAR1, osjar1-2 and osjar1-3, to study the biological function of jasmonates in rice anthesis. The florets of both osjar1 alleles stayed open during anthesis because the lodicules, which control flower opening in rice, were not withering on time. Furthermore, dehiscence of the anthers filled with viable pollen, was impaired, resulting in lower fertility. In situ hybridization and promoter GUS transgenic analysis confirmed OsJAR1 expression in these floral tissues. Flower opening induced by exogenous applied methyl jasmonate was impaired in osjar1 plants and was restored in a complementation experiment with transgenics expressing a wild type copy of OsJAR1 controlled by a rice actin promoter. Biochemical analysis showed that OsJAR1 encoded an enzyme conjugating jasmonic acid (JA) to at least Ile, Leu, Met, Phe, Trp and Val and both osjar1 alleles had substantial reduction in content of JA-Ile, JA-Leu and JA-Val in florets. We conclude that OsJAR1 is a JA-amino acid synthetase that is required for optimal flower opening and closing and anther dehiscence in rice.

KW - Arabidopsis

KW - JA

KW - JA-Ile

KW - MeJA

KW - OsJAR1

KW - Rice

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U2 - 10.1007/s11103-014-0212-y

DO - 10.1007/s11103-014-0212-y

M3 - Article

C2 - 24947835

AN - SCOPUS:84906077072

SN - 0167-4412

VL - 86

SP - 19

EP - 33

JO - Plant molecular biology

JF - Plant molecular biology

IS - 1-2

ER -

OsJAR1 is required for JA-regulated floret opening and anther dehiscence in rice

Abstract

Bibliographical note

Keywords

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