Oxygen sensing for in-vivo imaging by photoacoustic lifetime Probing

S. Ashkenazi, S. W. Huang, T. Horvath, Y. E L Koo, R. Kopelman

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

We have developed a new method to perform local measurements of fluorophore excited state lifetimes in turbid media without collecting the fluorescence emission. The method is based on a double pulse illumination where a first laser pulse excites the dye and then a second laser is used for photoacoustic probing of the transient absorption. The photoacoustic response generated by the probe pulse is recorded by an ultrasound receiver. Varying the time delay between excitation and probing allows for tracking the relaxation dynamics of the excited state. The method was validated by measuring the lifetime of an oxygen sensitive dye (Pt(II) octaethylporphine) solution at different concentrations of dissolved oxygen. The dye was excited with a 532 nm pulsed laser and the transient absorption at 740 nm was probed using a second pulsed laser system. The photoacoustics based results coincide with those obtained from simultaneous time-resolved fluorescent measurements. The method can be extended to photoacoustic lifetime imaging by using a receiver array instead of a single receiver. This opens unique possibilities for non-invasive, clinical functional imaging. For example, combined with oxygen sensitive dye, 3D imaging of tissue oxygenation could be developed for accurate diagnosis of cancer tumors, better planning of radiation therapy, and monitoring efficacy of treatment. Other potential applications include: in-vivo mapping of ion (e.g. Ca) concentration and dynamics and imaging of enzymes activity and metabolic functions, as well as environmental studies in turbid media.

Original languageEnglish (US)
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2008: The Ninth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics
DOIs
StatePublished - 2008
Event9th Conference on Photons Plus Ultrasound: Imaging and Sensing 2008 - San Jose, CA, United States
Duration: Jan 20 2008Jan 23 2008

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6856
ISSN (Print)1605-7422

Other

Other9th Conference on Photons Plus Ultrasound: Imaging and Sensing 2008
CountryUnited States
CitySan Jose, CA
Period1/20/081/23/08

Keywords

  • Fluorescence lifetime imaging
  • Molecular imaging
  • Oxygen sensing
  • Photoacoustic imaging
  • Transient absorption

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