OxyR contributes to the virulence of a Clonal Group A Escherichia coli strain (O17: K+: H18) in animal models of urinary tract infection, subcutaneous infection, and systemic sepsis

James R. Johnson; Thomas A. Russo; Sarah M. Drawz; Connie Clabots; Ruth Olson; Michael A. Kuskowski; Henry Rosen

doi:10.1016/j.micpath.2013.07.001

OxyR contributes to the virulence of a Clonal Group A Escherichia coli strain (O17: K+: H18) in animal models of urinary tract infection, subcutaneous infection, and systemic sepsis

James R. Johnson, Thomas A. Russo, Sarah M. Drawz, Connie Clabots, Ruth Olson, Michael A. Kuskowski, Henry Rosen

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The oxidative stress response regulator OxyR was assessed as both a urinary and extra-urinary virulence factor in Escherichia coli strain UCB34 (O17:K+:H18), a representative of the emergent Clonal Group A (CGA). Compared to UCB34, the isogenic oxyR mutant exhibited increased H₂O₂ sensitivity, indistinguishable invitro growth, and attenuated virulence in rodent models of urinary tract, subcutaneous infection, and systemic sepsis. Complemented mutants showed virulence levels comparable to parent strains in all models. These findings uniquely fulfill molecular Koch's postulates for a putative virulence factor of CGA, provide experimental evidence of an extra-urinary virulence promoting trait in CGA, and document a role for OxyR in local and systemic extra-urinary E.coli infections.

Original language	English (US)
Pages (from-to)	1-5
Number of pages	5
Journal	Microbial Pathogenesis
Volume	64
DOIs	https://doi.org/10.1016/j.micpath.2013.07.001
State	Published - Nov 2013

Bibliographical note

Funding Information:
This material is based upon work supported by Office of Research and Development, Medical Research Service, Department of Veterans Affairs (J.R.J., T.A.R.). Dave Prentiss (Minneapolis VA Medical Center) prepared the figures. Charles Dozois (INRS-Institut Armand-Frappier, Laval, Québec, Canada) provided the lac-derivative of the UCB717 parent strain.

Keywords

Clonal Group A
Escherichia coli infections
Extra-urinary infections
OxyR
Urinary tract infections
Virulence

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title = "OxyR contributes to the virulence of a Clonal Group A Escherichia coli strain (O17: K+: H18) in animal models of urinary tract infection, subcutaneous infection, and systemic sepsis",

abstract = "The oxidative stress response regulator OxyR was assessed as both a urinary and extra-urinary virulence factor in Escherichia coli strain UCB34 (O17:K+:H18), a representative of the emergent Clonal Group A (CGA). Compared to UCB34, the isogenic oxyR mutant exhibited increased H2O2 sensitivity, indistinguishable invitro growth, and attenuated virulence in rodent models of urinary tract, subcutaneous infection, and systemic sepsis. Complemented mutants showed virulence levels comparable to parent strains in all models. These findings uniquely fulfill molecular Koch's postulates for a putative virulence factor of CGA, provide experimental evidence of an extra-urinary virulence promoting trait in CGA, and document a role for OxyR in local and systemic extra-urinary E.coli infections.",

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author = "Johnson, {James R.} and Russo, {Thomas A.} and Drawz, {Sarah M.} and Connie Clabots and Ruth Olson and Kuskowski, {Michael A.} and Henry Rosen",

note = "Funding Information: This material is based upon work supported by Office of Research and Development, Medical Research Service, Department of Veterans Affairs (J.R.J., T.A.R.). Dave Prentiss (Minneapolis VA Medical Center) prepared the figures. Charles Dozois (INRS-Institut Armand-Frappier, Laval, Qu{\'e}bec, Canada) provided the lac-derivative of the UCB717 parent strain.",

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AU - Olson, Ruth

AU - Kuskowski, Michael A.

AU - Rosen, Henry

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KW - Virulence

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OxyR contributes to the virulence of a Clonal Group A Escherichia coli strain (O17: K+: H18) in animal models of urinary tract infection, subcutaneous infection, and systemic sepsis

Abstract

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Keywords

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