Partitioning and Accumulation of Perfluoroalkyl Substances in Model Lipid Bilayers and Bacteria

Nicole J.M. Fitzgerald, Andreas Wargenau, Carlise Sorenson, Joel Pedersen, Nathalie Tufenkji, Paige J. Novak, Matt F. Simcik

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Perfluoroalkyl substances (PFAS) are ubiquitous and persistent environmental contaminants, yet knowledge of their biological effects and mechanisms of action is limited. The highest aqueous PFAS concentrations are found in areas where bacteria are relied upon for functions such as nutrient cycling and contaminant degradation, including fire-training areas, wastewater treatment plants, and landfill leachates. This research sought to elucidate one of the mechanisms of action of PFAS by studying their uptake by bacteria and partitioning into model phospholipid bilayer membranes. PFAS partitioned into bacteria as well as model membranes (phospholipid liposomes and bilayers). The extent of incorporation into model membranes and bacteria was positively correlated to the number of fluorinated carbons. Furthermore, incorporation was greater for perfluorinated sulfonates than for perfluorinated carboxylates. Changes in zeta potential were observed in liposomes but not bacteria, consistent with PFAS being incorporated into the phospholipid bilayer membrane. Complementary to these results, PFAS were also found to alter the gel-to-fluid phase transition temperature of phospholipid bilayers, demonstrating that PFAS affected lateral phospholipid interactions. This investigation compliments other studies showing that sulfonated PFAS and PFAS with more than seven fluorinated carbons have a higher potential to accumulate within biota than carboxylated and shorter-chain PFAS.

Original languageEnglish (US)
Pages (from-to)10433-10440
Number of pages8
JournalEnvironmental Science and Technology
Volume52
Issue number18
DOIs
StatePublished - Sep 18 2018

Bibliographical note

Funding Information:
We thank Dr. Eric Stabb for the strain of A. f ischeri. Dr. Lisa Prevette at the University of Saint Thomas was also instrumental in assisting with liposome formation and analysis. This research was supported by the Environment and Natural Resources Trust Fund as recommended by the Legislative Citizen Commission on Minnesota Resources. N.T. acknowledges the Canada Research Chairs program and the Natural Sciences and Engineering Research Council of Canada.

Publisher Copyright:
© 2018 American Chemical Society.

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