Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats

Jae Kyeom Kim; Daniel D. Gallaher; Chi Chen; Cynthia M. Gallaher; Dan Yao; Sabrina P. Trudo

doi:10.1002/mnfr.201500790

Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats

Jae Kyeom Kim, Daniel D. Gallaher, Chi Chen, Cynthia M. Gallaher, Dan Yao, Sabrina P. Trudo

Food Science and Nutrition

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Scope: We previously showed that apiaceous but not cruciferous vegetables reduced DNA adducts formed by 2-amino-1-methyl-6-phenylimidazo[4, 5-b]pyridine (PhIP) in rats. Here, we report the effects of the putative chemopreventive phytochemicals from these vegetables on PhIP metabolism and DNA adduct formation. Methods and results: Rats received three supplemented diets: P + I (phenethyl isothiocyanate and indole-3-carbinol), furanocoumarins (FC, 5-methoxypsoralen, 8-methoxypsoralen, and isopimpinellin), and combination (P + I and FC). Phytochemical supplementation matched the levels in vegetables fed in our previous study. After 6 days, rats were injected with PhIP (10 mg/kg body wt) and killed after 24-h urine collection. Compared to the control, P + I increased activity of hepatic cytochrome P450 (CYP) 1A1 (10.1-fold), CYP1A2 (3.62-fold), and sulfotransferase 1A1 (2.70-fold). The combination diet also increased CYP1A1 and CYP1A2 activity. Urinary metabolomics revealed that PhIP metabolite profiles generally agreed with biotransformation enzyme activities. P + I and combination diets reduced PhIP-DNA adducts by 43.5 and 24.1%, respectively, whereas FC had no effect on adducts, compared to the control diet. Conclusion: Effects of phytochemicals on metabolic outcomes and markers of carcinogenesis might differ from fresh vegetables, thus limiting the inferences that one can draw from the effects of purified phytochemicals on the health benefits of the vegetables from which they derive.

Original language	English (US)
Pages (from-to)	1956-1966
Number of pages	11
Journal	Molecular Nutrition and Food Research
Volume	60
Issue number	9
DOIs	https://doi.org/10.1002/mnfr.201500790
State	Published - Sep 1 2016

Bibliographical note

Funding Information:
S.P.T. and D.D.G. designed the research; J.K.K., C.M.G., D.Y., D.D.G., and S.P.T. conducted the research; J.K.K., D.D.G., and C.C. analyzed the data; J.K.K. and S.P.T. drafted the manuscript; J.K.K., D.D.G., and S.P.T. had primary responsibility for the final content. All authors read and approved the final manuscript. This work was supported by Healthy Foods, Healthy Lives Institute, University of Minnesota (J.K.K.), NCI K07 CA128952 (S.P.T.), and the Analytical Biochemistry Shared Resource at the Masonic Cancer Center that is supported by NIH P30 CA077598. We would like to thank Dr. Robert Turesky for his expert guidance and resources.

Publisher Copyright:
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

Keywords

2-Amino-1-methyl-6-phenylimidazo[4, 5-b]pyridine
DNA adducts
Furanocoumarins
Indole-3-carbinol
Phenethyl isothiocyanate

Access

10.1002/mnfr.201500790

Fingerprint Dive into the research topics of 'Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats'. Together they form a unique fingerprint.

Cite this

Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats. / Kim, Jae Kyeom; Gallaher, Daniel D.; Chen, Chi ; Gallaher, Cynthia M.; Yao, Dan; Trudo, Sabrina P.

In: Molecular Nutrition and Food Research, Vol. 60, No. 9, 01.09.2016, p. 1956-1966.

Research output: Contribution to journal › Article › peer-review

@article{d06d20286a1c4a37aefb70a447fa1883,

title = "Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats",

abstract = "Scope: We previously showed that apiaceous but not cruciferous vegetables reduced DNA adducts formed by 2-amino-1-methyl-6-phenylimidazo[4, 5-b]pyridine (PhIP) in rats. Here, we report the effects of the putative chemopreventive phytochemicals from these vegetables on PhIP metabolism and DNA adduct formation. Methods and results: Rats received three supplemented diets: P + I (phenethyl isothiocyanate and indole-3-carbinol), furanocoumarins (FC, 5-methoxypsoralen, 8-methoxypsoralen, and isopimpinellin), and combination (P + I and FC). Phytochemical supplementation matched the levels in vegetables fed in our previous study. After 6 days, rats were injected with PhIP (10 mg/kg body wt) and killed after 24-h urine collection. Compared to the control, P + I increased activity of hepatic cytochrome P450 (CYP) 1A1 (10.1-fold), CYP1A2 (3.62-fold), and sulfotransferase 1A1 (2.70-fold). The combination diet also increased CYP1A1 and CYP1A2 activity. Urinary metabolomics revealed that PhIP metabolite profiles generally agreed with biotransformation enzyme activities. P + I and combination diets reduced PhIP-DNA adducts by 43.5 and 24.1%, respectively, whereas FC had no effect on adducts, compared to the control diet. Conclusion: Effects of phytochemicals on metabolic outcomes and markers of carcinogenesis might differ from fresh vegetables, thus limiting the inferences that one can draw from the effects of purified phytochemicals on the health benefits of the vegetables from which they derive.",

keywords = "2-Amino-1-methyl-6-phenylimidazo[4, 5-b]pyridine, DNA adducts, Furanocoumarins, Indole-3-carbinol, Phenethyl isothiocyanate",

author = "Kim, {Jae Kyeom} and Gallaher, {Daniel D.} and Chi Chen and Gallaher, {Cynthia M.} and Dan Yao and Trudo, {Sabrina P.}",

note = "Funding Information: S.P.T. and D.D.G. designed the research; J.K.K., C.M.G., D.Y., D.D.G., and S.P.T. conducted the research; J.K.K., D.D.G., and C.C. analyzed the data; J.K.K. and S.P.T. drafted the manuscript; J.K.K., D.D.G., and S.P.T. had primary responsibility for the final content. All authors read and approved the final manuscript. This work was supported by Healthy Foods, Healthy Lives Institute, University of Minnesota (J.K.K.), NCI K07 CA128952 (S.P.T.), and the Analytical Biochemistry Shared Resource at the Masonic Cancer Center that is supported by NIH P30 CA077598. We would like to thank Dr. Robert Turesky for his expert guidance and resources. Publisher Copyright: {\textcopyright} 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

year = "2016",

month = sep,

day = "1",

doi = "10.1002/mnfr.201500790",

language = "English (US)",

volume = "60",

pages = "1956--1966",

journal = "Molecular Nutrition and Food Research",

issn = "1613-4125",

publisher = "Wiley-VCH Verlag",

number = "9",

}

TY - JOUR

T1 - Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats

AU - Kim, Jae Kyeom

AU - Gallaher, Daniel D.

AU - Chen, Chi

AU - Gallaher, Cynthia M.

AU - Yao, Dan

AU - Trudo, Sabrina P.

N1 - Funding Information: S.P.T. and D.D.G. designed the research; J.K.K., C.M.G., D.Y., D.D.G., and S.P.T. conducted the research; J.K.K., D.D.G., and C.C. analyzed the data; J.K.K. and S.P.T. drafted the manuscript; J.K.K., D.D.G., and S.P.T. had primary responsibility for the final content. All authors read and approved the final manuscript. This work was supported by Healthy Foods, Healthy Lives Institute, University of Minnesota (J.K.K.), NCI K07 CA128952 (S.P.T.), and the Analytical Biochemistry Shared Resource at the Masonic Cancer Center that is supported by NIH P30 CA077598. We would like to thank Dr. Robert Turesky for his expert guidance and resources. Publisher Copyright: © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2016/9/1

Y1 - 2016/9/1

N2 - Scope: We previously showed that apiaceous but not cruciferous vegetables reduced DNA adducts formed by 2-amino-1-methyl-6-phenylimidazo[4, 5-b]pyridine (PhIP) in rats. Here, we report the effects of the putative chemopreventive phytochemicals from these vegetables on PhIP metabolism and DNA adduct formation. Methods and results: Rats received three supplemented diets: P + I (phenethyl isothiocyanate and indole-3-carbinol), furanocoumarins (FC, 5-methoxypsoralen, 8-methoxypsoralen, and isopimpinellin), and combination (P + I and FC). Phytochemical supplementation matched the levels in vegetables fed in our previous study. After 6 days, rats were injected with PhIP (10 mg/kg body wt) and killed after 24-h urine collection. Compared to the control, P + I increased activity of hepatic cytochrome P450 (CYP) 1A1 (10.1-fold), CYP1A2 (3.62-fold), and sulfotransferase 1A1 (2.70-fold). The combination diet also increased CYP1A1 and CYP1A2 activity. Urinary metabolomics revealed that PhIP metabolite profiles generally agreed with biotransformation enzyme activities. P + I and combination diets reduced PhIP-DNA adducts by 43.5 and 24.1%, respectively, whereas FC had no effect on adducts, compared to the control diet. Conclusion: Effects of phytochemicals on metabolic outcomes and markers of carcinogenesis might differ from fresh vegetables, thus limiting the inferences that one can draw from the effects of purified phytochemicals on the health benefits of the vegetables from which they derive.

AB - Scope: We previously showed that apiaceous but not cruciferous vegetables reduced DNA adducts formed by 2-amino-1-methyl-6-phenylimidazo[4, 5-b]pyridine (PhIP) in rats. Here, we report the effects of the putative chemopreventive phytochemicals from these vegetables on PhIP metabolism and DNA adduct formation. Methods and results: Rats received three supplemented diets: P + I (phenethyl isothiocyanate and indole-3-carbinol), furanocoumarins (FC, 5-methoxypsoralen, 8-methoxypsoralen, and isopimpinellin), and combination (P + I and FC). Phytochemical supplementation matched the levels in vegetables fed in our previous study. After 6 days, rats were injected with PhIP (10 mg/kg body wt) and killed after 24-h urine collection. Compared to the control, P + I increased activity of hepatic cytochrome P450 (CYP) 1A1 (10.1-fold), CYP1A2 (3.62-fold), and sulfotransferase 1A1 (2.70-fold). The combination diet also increased CYP1A1 and CYP1A2 activity. Urinary metabolomics revealed that PhIP metabolite profiles generally agreed with biotransformation enzyme activities. P + I and combination diets reduced PhIP-DNA adducts by 43.5 and 24.1%, respectively, whereas FC had no effect on adducts, compared to the control diet. Conclusion: Effects of phytochemicals on metabolic outcomes and markers of carcinogenesis might differ from fresh vegetables, thus limiting the inferences that one can draw from the effects of purified phytochemicals on the health benefits of the vegetables from which they derive.

KW - 2-Amino-1-methyl-6-phenylimidazo[4, 5-b]pyridine

KW - DNA adducts

KW - Furanocoumarins

KW - Indole-3-carbinol

KW - Phenethyl isothiocyanate

UR - http://www.scopus.com/inward/record.url?scp=85028237064&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85028237064&partnerID=8YFLogxK

U2 - 10.1002/mnfr.201500790

DO - 10.1002/mnfr.201500790

M3 - Article

C2 - 27133590

AN - SCOPUS:85028237064

VL - 60

SP - 1956

EP - 1966

JO - Molecular Nutrition and Food Research

JF - Molecular Nutrition and Food Research

SN - 1613-4125

IS - 9

ER -

Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats

Abstract

Bibliographical note

Keywords

Access

Other files and links

Cite this