PiRNA-mediated transposon regulation and the germ-line mutation rate in Drosophila melanogaster males

Michael J. Simmons, Mark P. Peterson, Michael W. Thorp, Jared T. Buschette, Stephanie N. DiPrima, Christine L. Harter, Matthew J. Skolnick

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Transposons, especially retrotransposons, are abundant in the genome of Drosophila melanogaster. These mobile elements are regulated by small RNAs that interact with the Piwi family of proteins-the piwi-interacting or piRNAs. The Piwi proteins are encoded by the genes argonaute3 (ago3), aubergine (aub), and piwi. Heterochromatin Protein 1 (HP1), a chromatin-organizing protein encoded by the Suppressor of variegation 205 [. Su(var)205] gene, also plays a role in this regulation. To assess the mutational impact of weakening the system for transposon regulation, we measured the frequency of recessive X-linked lethal mutations occurring in the germ lines of males from stocks that were heterozygous for mutant alleles of the ago3, aub, piwi, or Su(var)205 genes. These mutant alleles are expected to deplete the wild-type proteins encoded by these genes by as much as 50%. The mutant alleles of piwi and Su(var)205 significantly increased the X-linked lethal mutation frequency, whereas the mutant alleles of ago3 did not. An increased mutation frequency was also observed in males from one of two mutant aub stocks, but this increase may not have been due to the aub mutant. The increased mutation frequency caused by depleting Piwi or HP1suggests that chromatin-organizing proteins play important roles in minimizing the germ-line mutation rate, possibly by stabilizing the structure of the heterochromatin in which many transposons are situated.

Original languageEnglish (US)
Pages (from-to)16-21
Number of pages6
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume773
DOIs
StatePublished - Mar 1 2015

Keywords

  • Chromatin organization
  • Mutation rate
  • PiRNA
  • Transposons

Fingerprint Dive into the research topics of 'PiRNA-mediated transposon regulation and the germ-line mutation rate in Drosophila melanogaster males'. Together they form a unique fingerprint.

Cite this