TY - JOUR
T1 - Platelet adhesion to novel phospholipid materials
T2 - Modified phosphatidylcholine covalently immobilized to silica, polypropylene, and PTFE materials
AU - Köhler, A. S.
AU - Parks, P. J.
AU - Mooradian, D. L.
AU - Rao, G. H.R.
AU - Furcht, L. T.
PY - 1996/10
Y1 - 1996/10
N2 - Based on the premise of achieving blood compatibility through mimicking the chemical constituents of the biologically inert surface of the unactivated platelet membrane, a process was developed that entails the covalent grafting of modified phosphatidylcholine molecules to materials including silica, polypropylene, and polytetrafluoroethylene (PTFE) polymer films. These materials were characterized using x-ray photoelectron spectroscopy (XPS) and contact-angle measurements. The phosphatidylcholine- containing materials (PC materials) were used as substrates in the platelet- adhesion assays and were subjected to enzymatic degradation evaluation. Phosphatidylcholine-grafted silica materials do not support platelet adhesion. In addition the number of adherent platelets correlate with the amount of grafted phospholipid present, as indicated by the phosphorus/carbon ratio obtained by XPS analysis. Platelet adhesion to phosphatidylcholine- grafted polypropylene and PTFE was inhibited 80% and 90%, respectively, when compared with platelet adhesion to unmodified polypropylene and PTFE.
AB - Based on the premise of achieving blood compatibility through mimicking the chemical constituents of the biologically inert surface of the unactivated platelet membrane, a process was developed that entails the covalent grafting of modified phosphatidylcholine molecules to materials including silica, polypropylene, and polytetrafluoroethylene (PTFE) polymer films. These materials were characterized using x-ray photoelectron spectroscopy (XPS) and contact-angle measurements. The phosphatidylcholine- containing materials (PC materials) were used as substrates in the platelet- adhesion assays and were subjected to enzymatic degradation evaluation. Phosphatidylcholine-grafted silica materials do not support platelet adhesion. In addition the number of adherent platelets correlate with the amount of grafted phospholipid present, as indicated by the phosphorus/carbon ratio obtained by XPS analysis. Platelet adhesion to phosphatidylcholine- grafted polypropylene and PTFE was inhibited 80% and 90%, respectively, when compared with platelet adhesion to unmodified polypropylene and PTFE.
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U2 - 10.1002/(SICI)1097-4636(199610)32:2<237::AID-JBM13>3.0.CO;2-G
DO - 10.1002/(SICI)1097-4636(199610)32:2<237::AID-JBM13>3.0.CO;2-G
M3 - Article
C2 - 8884501
AN - SCOPUS:0030270005
SN - 0021-9304
VL - 32
SP - 237
EP - 242
JO - Journal of Biomedical Materials Research
JF - Journal of Biomedical Materials Research
IS - 2
ER -