Poly (lactic acid)-chitosan-collagen composite nanofibers as substrates for blood outgrowth endothelial cells

B. Swarnalatha; S. L. Nair; K. T. Shalumon; L. C. Milbauer; R. Jayakumar; B. Paul-Prasanth; K. K. Menon; R. P. Hebbel; A. Somani; Shantikumar V. Nair

doi:10.1016/j.ijbiomac.2013.03.060

Poly (lactic acid)-chitosan-collagen composite nanofibers as substrates for blood outgrowth endothelial cells

B. Swarnalatha, S. L. Nair, K. T. Shalumon, L. C. Milbauer, R. Jayakumar, B. Paul-Prasanth, K. K. Menon, R. P. Hebbel, A. Somani, Shantikumar V. Nair

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25 Scopus citations

Abstract

In this work, the attachment, viability and functionality of rat Blood Outgrowth Endothelial Cells (rBOEC) and genetically modified rBOEC (rBOEC/eNOS-GFP), which over express endothelial nitric oxide synthase (eNOS), were investigated on Poly(lactic acid) (PLA)-chitosan and PLA-chitosan-collagen nanofibrous scaffolds. Both the cell types displayed good attachment, remained viable and functional on both scaffolds. Moreover, incorporation of collagen in the scaffold helped in sustaining the rBOEC for upto one week, although collagen was not found necessary for rBOEC/eNOS-GFP. We conclude that PLA-chitosan based nanofibrous scaffolds can be a potential candidate for BOEC based wound healing applications.

Original language	English (US)
Pages (from-to)	220-224
Number of pages	5
Journal	International Journal of Biological Macromolecules
Volume	58
DOIs	https://doi.org/10.1016/j.ijbiomac.2013.03.060
State	Published - Jul 2013

Bibliographical note

Funding Information:
The authors are thankful to the Indian Council for Medical Research (ICMR) , Government of India as well as to the University of Minnesota (UMN) for grant funding (ICMR-UMN Joint Initiative, Ref. ID: 5/3/8/3/UOM/2011-RHN). The authors are grateful to Amrita Vishwa Vidyapeetham for providing the infrastructural support for this work.

Keywords

BOEC
Chitosan
Collagen
ENOS
Nanofibers
Poly (lactic acid)
Wound healing

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Swarnalatha, B., Nair, S. L., Shalumon, K. T., Milbauer, L. C., Jayakumar, R., Paul-Prasanth, B., Menon, K. K., Hebbel, R. P., Somani, A., & Nair, S. V. (2013). Poly (lactic acid)-chitosan-collagen composite nanofibers as substrates for blood outgrowth endothelial cells. International Journal of Biological Macromolecules, 58, 220-224. https://doi.org/10.1016/j.ijbiomac.2013.03.060

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abstract = "In this work, the attachment, viability and functionality of rat Blood Outgrowth Endothelial Cells (rBOEC) and genetically modified rBOEC (rBOEC/eNOS-GFP), which over express endothelial nitric oxide synthase (eNOS), were investigated on Poly(lactic acid) (PLA)-chitosan and PLA-chitosan-collagen nanofibrous scaffolds. Both the cell types displayed good attachment, remained viable and functional on both scaffolds. Moreover, incorporation of collagen in the scaffold helped in sustaining the rBOEC for upto one week, although collagen was not found necessary for rBOEC/eNOS-GFP. We conclude that PLA-chitosan based nanofibrous scaffolds can be a potential candidate for BOEC based wound healing applications.",

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AU - Milbauer, L. C.

AU - Jayakumar, R.

AU - Paul-Prasanth, B.

AU - Menon, K. K.

AU - Hebbel, R. P.

AU - Somani, A.

AU - Nair, Shantikumar V.

N1 - Funding Information: The authors are thankful to the Indian Council for Medical Research (ICMR) , Government of India as well as to the University of Minnesota (UMN) for grant funding (ICMR-UMN Joint Initiative, Ref. ID: 5/3/8/3/UOM/2011-RHN). The authors are grateful to Amrita Vishwa Vidyapeetham for providing the infrastructural support for this work.

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N2 - In this work, the attachment, viability and functionality of rat Blood Outgrowth Endothelial Cells (rBOEC) and genetically modified rBOEC (rBOEC/eNOS-GFP), which over express endothelial nitric oxide synthase (eNOS), were investigated on Poly(lactic acid) (PLA)-chitosan and PLA-chitosan-collagen nanofibrous scaffolds. Both the cell types displayed good attachment, remained viable and functional on both scaffolds. Moreover, incorporation of collagen in the scaffold helped in sustaining the rBOEC for upto one week, although collagen was not found necessary for rBOEC/eNOS-GFP. We conclude that PLA-chitosan based nanofibrous scaffolds can be a potential candidate for BOEC based wound healing applications.

AB - In this work, the attachment, viability and functionality of rat Blood Outgrowth Endothelial Cells (rBOEC) and genetically modified rBOEC (rBOEC/eNOS-GFP), which over express endothelial nitric oxide synthase (eNOS), were investigated on Poly(lactic acid) (PLA)-chitosan and PLA-chitosan-collagen nanofibrous scaffolds. Both the cell types displayed good attachment, remained viable and functional on both scaffolds. Moreover, incorporation of collagen in the scaffold helped in sustaining the rBOEC for upto one week, although collagen was not found necessary for rBOEC/eNOS-GFP. We conclude that PLA-chitosan based nanofibrous scaffolds can be a potential candidate for BOEC based wound healing applications.

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Poly (lactic acid)-chitosan-collagen composite nanofibers as substrates for blood outgrowth endothelial cells

Abstract

Bibliographical note

Keywords

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