Polymerase chain reaction for diagnosis of porcine proliferative enteropathy

Steven McOrist, Connie J. Gebhart, Gordon H.K. Lawson

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

A polymerase chain reaction (PCR) assay for detection of the intracellular bacteria, ileal symbiont intracellularis of porcine proliferative enteropathy is described. The testis based on specific DNA primers and gave positive PCR product from samples of preserved intestinal mucosa and faeces from affected pigs. Mucosa and faeces from normal pigs gave no positive PCR products. The identity of the PCR product was confirmed by DNA-DNA hybridization with a probe, pCLO78, specific for IS intracellularis. Positive results were only observed in animals with active lesions of proliferative enteropathy. PCR is probably the most useful method for diagnosis of prolifrative enteropathy that is currently available for live animals.

Original languageEnglish (US)
Pages (from-to)205-212
Number of pages8
JournalVeterinary Microbiology
Volume41
Issue number3
DOIs
StatePublished - Aug 1 1994

Keywords

  • Diagnosis, bacteria general
  • PCR
  • Pig
  • Proliferative enterophaty

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