Polymerase chain reaction for diagnosis of porcine proliferative enteropathy

Steven McOrist; Connie J. Gebhart; Gordon H.K. Lawson

doi:10.1016/0378-1135(94)90101-5

Polymerase chain reaction for diagnosis of porcine proliferative enteropathy

Steven McOrist, Connie J. Gebhart, Gordon H.K. Lawson

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Abstract

A polymerase chain reaction (PCR) assay for detection of the intracellular bacteria, ileal symbiont intracellularis of porcine proliferative enteropathy is described. The testis based on specific DNA primers and gave positive PCR product from samples of preserved intestinal mucosa and faeces from affected pigs. Mucosa and faeces from normal pigs gave no positive PCR products. The identity of the PCR product was confirmed by DNA-DNA hybridization with a probe, pCLO78, specific for IS intracellularis. Positive results were only observed in animals with active lesions of proliferative enteropathy. PCR is probably the most useful method for diagnosis of prolifrative enteropathy that is currently available for live animals.

Original language	English (US)
Pages (from-to)	205-212
Number of pages	8
Journal	Veterinary Microbiology
Volume	41
Issue number	3
DOIs	https://doi.org/10.1016/0378-1135(94)90101-5
State	Published - Aug 1 1994

Bibliographical note

Funding Information:
We thank Gary Jones, Barbara Swanson, Barbara Tall, Tudor Lloyd and Neil MacIntyre for their valuable input into this work, which was partly supported by the Agricultural Food Research Council and the Wellcome Trust of the UK.

Keywords

Diagnosis, bacteria general
PCR
Pig
Proliferative enterophaty

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title = "Polymerase chain reaction for diagnosis of porcine proliferative enteropathy",

abstract = "A polymerase chain reaction (PCR) assay for detection of the intracellular bacteria, ileal symbiont intracellularis of porcine proliferative enteropathy is described. The testis based on specific DNA primers and gave positive PCR product from samples of preserved intestinal mucosa and faeces from affected pigs. Mucosa and faeces from normal pigs gave no positive PCR products. The identity of the PCR product was confirmed by DNA-DNA hybridization with a probe, pCLO78, specific for IS intracellularis. Positive results were only observed in animals with active lesions of proliferative enteropathy. PCR is probably the most useful method for diagnosis of prolifrative enteropathy that is currently available for live animals.",

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note = "Funding Information: We thank Gary Jones, Barbara Swanson, Barbara Tall, Tudor Lloyd and Neil MacIntyre for their valuable input into this work, which was partly supported by the Agricultural Food Research Council and the Wellcome Trust of the UK.",

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AU - Gebhart, Connie J.

AU - Lawson, Gordon H.K.

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N2 - A polymerase chain reaction (PCR) assay for detection of the intracellular bacteria, ileal symbiont intracellularis of porcine proliferative enteropathy is described. The testis based on specific DNA primers and gave positive PCR product from samples of preserved intestinal mucosa and faeces from affected pigs. Mucosa and faeces from normal pigs gave no positive PCR products. The identity of the PCR product was confirmed by DNA-DNA hybridization with a probe, pCLO78, specific for IS intracellularis. Positive results were only observed in animals with active lesions of proliferative enteropathy. PCR is probably the most useful method for diagnosis of prolifrative enteropathy that is currently available for live animals.

AB - A polymerase chain reaction (PCR) assay for detection of the intracellular bacteria, ileal symbiont intracellularis of porcine proliferative enteropathy is described. The testis based on specific DNA primers and gave positive PCR product from samples of preserved intestinal mucosa and faeces from affected pigs. Mucosa and faeces from normal pigs gave no positive PCR products. The identity of the PCR product was confirmed by DNA-DNA hybridization with a probe, pCLO78, specific for IS intracellularis. Positive results were only observed in animals with active lesions of proliferative enteropathy. PCR is probably the most useful method for diagnosis of prolifrative enteropathy that is currently available for live animals.

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Polymerase chain reaction for diagnosis of porcine proliferative enteropathy

Abstract

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