Potential use of lymphocyte blastogenic responses in diagnosis of bovine tuberculosis

Charles O. Thoen; Jerald L. Jarnagin; Charles C Muscoplat; L. S. Cram; Donald W. Johnson; Rube Harrington

doi:10.1016/0147-9571(80)90012-0

Potential use of lymphocyte blastogenic responses in diagnosis of bovine tuberculosis

Charles O. Thoen, Jerald L. Jarnagin, Charles C Muscoplat, L. S. Cram, Donald W. Johnson, Rube Harrington

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Abstract

A comparison of in vitro lymphocyte responses and delayed type tuberculin skin test responses was made in an animal experimentally exposed to a Mycobacterium bovis-infected animal and in cattle naturally infected with M. bovis. Tuberculin skin tests did not suppress in vitro lymphocyte responses to M. bovis PPD and to M. avium PPD tuberculin. The whole blood test used in these studies provided for considerable savings in time as compared to use of purified lymphocytes for evaluating in vitro cellular responses. Variations in the responsiveness of lymphocytes to specific mycobacterial antigens was observed, therefore, it is recommended that profiles be established using three or more tests conducted at 14-day intervals.

Original language	English (US)
Pages (from-to)	355-361
Number of pages	7
Journal	Comparative Immunology, Microbiology and Infectious Diseases
Volume	3
Issue number	3
DOIs	https://doi.org/10.1016/0147-9571(80)90012-0
State	Published - 1980

Keywords

In vitro lymphocyte responses
Mycobacterium avium PPD
Mycobacterium bovis PPD
cattle
tuberculin skin tests

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title = "Potential use of lymphocyte blastogenic responses in diagnosis of bovine tuberculosis",

abstract = "A comparison of in vitro lymphocyte responses and delayed type tuberculin skin test responses was made in an animal experimentally exposed to a Mycobacterium bovis-infected animal and in cattle naturally infected with M. bovis. Tuberculin skin tests did not suppress in vitro lymphocyte responses to M. bovis PPD and to M. avium PPD tuberculin. The whole blood test used in these studies provided for considerable savings in time as compared to use of purified lymphocytes for evaluating in vitro cellular responses. Variations in the responsiveness of lymphocytes to specific mycobacterial antigens was observed, therefore, it is recommended that profiles be established using three or more tests conducted at 14-day intervals.",

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T1 - Potential use of lymphocyte blastogenic responses in diagnosis of bovine tuberculosis

AU - Thoen, Charles O.

AU - Jarnagin, Jerald L.

AU - Muscoplat, Charles C

AU - Cram, L. S.

AU - Johnson, Donald W.

AU - Harrington, Rube

PY - 1980

Y1 - 1980

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AB - A comparison of in vitro lymphocyte responses and delayed type tuberculin skin test responses was made in an animal experimentally exposed to a Mycobacterium bovis-infected animal and in cattle naturally infected with M. bovis. Tuberculin skin tests did not suppress in vitro lymphocyte responses to M. bovis PPD and to M. avium PPD tuberculin. The whole blood test used in these studies provided for considerable savings in time as compared to use of purified lymphocytes for evaluating in vitro cellular responses. Variations in the responsiveness of lymphocytes to specific mycobacterial antigens was observed, therefore, it is recommended that profiles be established using three or more tests conducted at 14-day intervals.

KW - In vitro lymphocyte responses

KW - Mycobacterium avium PPD

KW - Mycobacterium bovis PPD

KW - cattle

KW - tuberculin skin tests

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Potential use of lymphocyte blastogenic responses in diagnosis of bovine tuberculosis

Abstract

Keywords

UN SDGs

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