TY - JOUR
T1 - Preferential pairing of T and B cells for production of antibodies without covalent association of T and B epitopes
AU - Bellone, Matteo
AU - Karachunski, Peter I
AU - Ostlie, Norma
AU - Lei, Sijin
AU - Conti‐Tronconi, Bianca M.
PY - 1994/4
Y1 - 1994/4
N2 - T cell from H‐2b mice recognize at least 12 sequence regions on the Torpedo acetylcholine receptor (TAChR) α, γ and δ subunits. Immunization of C57BL/6 mice with individual synthetic TAChR sequences known to contain CD4+ epitopes resulted in most cases (10 out of 12 peptides) in anti‐peptide antibody (Ab) production, indicating that short TAChR sequences contain both CD4+ and B epitopes. Immunization of C57BL/6 mice with a mixture of a CD4+ epitope peptide, from the TAChR or from an unrelated protein, plus another TAChR sequence forming a “pure” B epitope (Tα63–80), induced in most cases anti‐peptide Ab and CD4+ cell sensitization only against the peptide containing the CD4+ epitope. However, when the T epitope peptide Tα360–378 was co‐injected with the B epitope, Ab were also produced against the B epitope peptide. Injection of the individual peptides Tα360–378 and Tα63–80 at different and distant sites along the back of mice elicited sensitization of CD4+ cells and Ab production only against peptide Tα360–378. Therefore, when optimal cooperation between T and B cells occurs, spatial proximity but not covalent association of the B and the CD4+ epitope is necessary for production of Ab against the B epitope.
AB - T cell from H‐2b mice recognize at least 12 sequence regions on the Torpedo acetylcholine receptor (TAChR) α, γ and δ subunits. Immunization of C57BL/6 mice with individual synthetic TAChR sequences known to contain CD4+ epitopes resulted in most cases (10 out of 12 peptides) in anti‐peptide antibody (Ab) production, indicating that short TAChR sequences contain both CD4+ and B epitopes. Immunization of C57BL/6 mice with a mixture of a CD4+ epitope peptide, from the TAChR or from an unrelated protein, plus another TAChR sequence forming a “pure” B epitope (Tα63–80), induced in most cases anti‐peptide Ab and CD4+ cell sensitization only against the peptide containing the CD4+ epitope. However, when the T epitope peptide Tα360–378 was co‐injected with the B epitope, Ab were also produced against the B epitope peptide. Injection of the individual peptides Tα360–378 and Tα63–80 at different and distant sites along the back of mice elicited sensitization of CD4+ cells and Ab production only against peptide Tα360–378. Therefore, when optimal cooperation between T and B cells occurs, spatial proximity but not covalent association of the B and the CD4+ epitope is necessary for production of Ab against the B epitope.
KW - Experimental autoimmune myasthenia gravis
KW - Nicotinic acetylcholine receptor
KW - Synthetic peptides
KW - T and B epitopes
KW - T‐B cell cooperation
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U2 - 10.1002/eji.1830240404
DO - 10.1002/eji.1830240404
M3 - Article
C2 - 7512029
AN - SCOPUS:0028349398
SN - 0014-2980
VL - 24
SP - 799
EP - 804
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 4
ER -