The enzyme protocatechuate 3,4-dioxygenase from the Gram positive organism Brevibacterium fuscum crystallizes in the triclinic space group P1 with unit cell dimensions a = 96.1 Å, b = 97.2 Å, c = 118.1 Å and α = 113.9°, β = 90.7°, γ = 117.8°. The rod-like crystals diffract to 2.4 Å resolution. Rotation function analysis suggests that there are six protomers arranged with local 32 symmetry in the asymmetric unit rather than the previously proposed pentameric complex.
- Brevibacterium fuscum
- X-ray crystallography