TY - JOUR
T1 - Preliminary crystallographic study of protocatechuate 3,4-Dioxygenase from Brevibacterium fuscum
AU - Earhart, Cathleen A.
AU - Radhakrishnan, R.
AU - Orville, Allen M.
AU - Lipscomb, John D.
AU - Ohlendorf, Douglas H.
PY - 1994/2/10
Y1 - 1994/2/10
N2 - The enzyme protocatechuate 3,4-dioxygenase from the Gram positive organism Brevibacterium fuscum crystallizes in the triclinic space group P1 with unit cell dimensions a = 96.1 Å, b = 97.2 Å, c = 118.1 Å and α = 113.9°, β = 90.7°, γ = 117.8°. The rod-like crystals diffract to 2.4 Å resolution. Rotation function analysis suggests that there are six protomers arranged with local 32 symmetry in the asymmetric unit rather than the previously proposed pentameric complex.
AB - The enzyme protocatechuate 3,4-dioxygenase from the Gram positive organism Brevibacterium fuscum crystallizes in the triclinic space group P1 with unit cell dimensions a = 96.1 Å, b = 97.2 Å, c = 118.1 Å and α = 113.9°, β = 90.7°, γ = 117.8°. The rod-like crystals diffract to 2.4 Å resolution. Rotation function analysis suggests that there are six protomers arranged with local 32 symmetry in the asymmetric unit rather than the previously proposed pentameric complex.
KW - Brevibacterium fuscum
KW - Crystallization
KW - Dioxygenase
KW - X-ray crystallography
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U2 - 10.1006/jmbi.1994.1143
DO - 10.1006/jmbi.1994.1143
M3 - Article
C2 - 8107119
AN - SCOPUS:0028260029
SN - 0022-2836
VL - 236
SP - 374
EP - 376
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 1
ER -