Preparation of sirna-encapsulated plga nanoparticles for sustained release of sirna and evaluation of encapsulation efficiency

Panayotis Pantazis; Konstantinos Dimas; James H. Wyche; Shrikant Anant; Courtney W. Houchen; Jayanth Panyam; Rama P. Ramanujam

doi:10.1007/978-1-61779-953-2_25

Preparation of sirna-encapsulated plga nanoparticles for sustained release of sirna and evaluation of encapsulation efficiency

Panayotis Pantazis, Konstantinos Dimas, James H. Wyche, Shrikant Anant, Courtney W. Houchen, Jayanth Panyam, Rama P. Ramanujam

Pharmaceutics

Research output: Chapter in Book/Report/Conference proceeding › Chapter

33 Scopus citations

Abstract

Nanoparticles (NPs) formulated using poly (d,l-lactide-co-glycolide) (PLGA), a biodegradable, biocompatible, and clinically approved polymer, have been widely used for targeted drug delivery. Here we provide methods for preparing PLGA NPs that encapsulate small interfering RNA (siRNA). The siRNA NPs are formulated using a double-emulsion solvent evaporation technique with the addition of a small amount of the cationic polymer, polyethyleneimine, which significantly increases siRNA encapsulation.

Original language	English (US)
Title of host publication	Nanoparticles in Biology and Medicine
Subtitle of host publication	Methods and Protocols
Editors	Mikhail Soloviev
Pages	311-319
Number of pages	9
DOIs	https://doi.org/10.1007/978-1-61779-953-2_25
State	Published - 2012

Publication series

Name	Methods in Molecular Biology
Volume	906
ISSN (Print)	1064-3745

Keywords

Cancer therapy
Cancer treatment
Drug delivery
Nanoparticles
Poly(lactic-co-glycolic) acid
RNAi
siRNA

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-61779-953-2_25

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Pantazis, P., Dimas, K., Wyche, J. H., Anant, S., Houchen, C. W., Panyam, J., & Ramanujam, R. P. (2012). Preparation of sirna-encapsulated plga nanoparticles for sustained release of sirna and evaluation of encapsulation efficiency. In M. Soloviev (Ed.), Nanoparticles in Biology and Medicine: Methods and Protocols (pp. 311-319). (Methods in Molecular Biology; Vol. 906). https://doi.org/10.1007/978-1-61779-953-2_25

Preparation of sirna-encapsulated plga nanoparticles for sustained release of sirna and evaluation of encapsulation efficiency. / Pantazis, Panayotis; Dimas, Konstantinos; Wyche, James H. et al.
Nanoparticles in Biology and Medicine: Methods and Protocols. ed. / Mikhail Soloviev. 2012. p. 311-319 (Methods in Molecular Biology; Vol. 906).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Pantazis, P, Dimas, K, Wyche, JH, Anant, S, Houchen, CW, Panyam, J & Ramanujam, RP 2012, Preparation of sirna-encapsulated plga nanoparticles for sustained release of sirna and evaluation of encapsulation efficiency. in M Soloviev (ed.), Nanoparticles in Biology and Medicine: Methods and Protocols. Methods in Molecular Biology, vol. 906, pp. 311-319. https://doi.org/10.1007/978-1-61779-953-2_25

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Preparation of sirna-encapsulated plga nanoparticles for sustained release of sirna and evaluation of encapsulation efficiency

Abstract

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Keywords

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