Preparation of sirna-encapsulated plga nanoparticles for sustained release of sirna and evaluation of encapsulation efficiency

Panayotis Pantazis, Konstantinos Dimas, James H. Wyche, Shrikant Anant, Courtney W. Houchen, Jayanth Panyam, Rama P. Ramanujam

Research output: Chapter in Book/Report/Conference proceedingChapter

19 Scopus citations

Abstract

Nanoparticles (NPs) formulated using poly (d,l-lactide-co-glycolide) (PLGA), a biodegradable, biocompatible, and clinically approved polymer, have been widely used for targeted drug delivery. Here we provide methods for preparing PLGA NPs that encapsulate small interfering RNA (siRNA). The siRNA NPs are formulated using a double-emulsion solvent evaporation technique with the addition of a small amount of the cationic polymer, polyethyleneimine, which significantly increases siRNA encapsulation.

Original languageEnglish (US)
Title of host publicationNanoparticles in Biology and Medicine
Subtitle of host publicationMethods and Protocols
EditorsMikhail Soloviev
Pages311-319
Number of pages9
DOIs
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
Volume906
ISSN (Print)1064-3745

Keywords

  • Cancer therapy
  • Cancer treatment
  • Drug delivery
  • Nanoparticles
  • Poly(lactic-co-glycolic) acid
  • RNAi
  • siRNA

Fingerprint Dive into the research topics of 'Preparation of sirna-encapsulated plga nanoparticles for sustained release of sirna and evaluation of encapsulation efficiency'. Together they form a unique fingerprint.

Cite this