Propagation and quantitation of animal herpesviruses in eight cell culture systems

Richard B. Peterson, Sagar M Goyal

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


A comparative study was carried out to determine the relative sensitivities of eight different cell culture systems to six different herpesviruses of animals. The cells used were: OFL (ovine fetal lung), ML (mink lung), FK (ferret kidney), PTK-2 (potoroo kidney), TEK (turkey embryo kidney), ED (equine dermal), BT (bovine turbinate), and PK15 (porcine kidney). The viruses tested were: PRV (pseudorabies) of swine, CPHV (caprine herpesvirus), IBRV (infectious bovine rhinotracheitis virus), DN-599 strain of bovine herpesvirus type 4, EHV-1 (equine herpesvirus), and CHV (canine herpesvirus). On the basis of virus titers obtained and the time of appearance of CPE (cytopathic effects), ML cells were found to be the most useful because of their sensitivity to all six viruses tested. BT and OFL cells were also found to be highly sensitive to all viruses with the exception of CHV.

Original languageEnglish (US)
Pages (from-to)93-98
Number of pages6
JournalComparative Immunology, Microbiology and Infectious Diseases
Issue number2
StatePublished - 1988


  • Pseudorabies virus
  • bovine herpesvirus type-4
  • canine herpesvirus
  • caprine herpesvirus
  • cell culture systems
  • equine rhinopneumonitis virus
  • infectious bovine rhinotracheitis virus

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