Protein phosphatase subunit G5PR is needed for inhibition of B cell receptor-induced apoptosis

Yan Xing, Hideya Igarashi, Xiaodan Wang, Nobuo Sakaguchi

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

B cell receptor (BCR) cross-linking induces B cell proliferation and sustains survival through the phosphorylation-dependent signals. We report that a loss of the protein phosphatase component G5PR increased the activation-induced cell death (AICD) and thus impaired B cell survival. G5PR associates with GANP, whose expression is up-regulated in mature B cells of the peripheral lymphoid organs. To study G5PR function, the G5pr gene was conditionally targeted with the CD19-Cre combination (G5pr-/- mice). The G5pr-/- mice had a decreased number of splenic B cells (60% of the controls). G5pr-/- B cells showed a normal proliferative response to lipopolysaccharide or anti-CD40 antibody stimulation but not to BCR cross-linking with or without IL-4 in vitro. G5pr-/- B cells did not show abnormalities in the BCR-mediated activation of Erks and NF-κB, cyclin D2 induction, or Akt activation. However, G5pr-/- B cells were sensitive to AICD caused by BCR cross-linking. This was associated with an increased depolarization of the mitochondrial membrane and the enhanced activation of c-Jun NH2-terminal protein kinase and Bim. These results suggest that G5PR is required for the BCR-mediated proliferation associated with the prevention of AICD in mature B cells. JEM

Original languageEnglish (US)
Pages (from-to)707-719
Number of pages13
JournalJournal of Experimental Medicine
Volume202
Issue number5
DOIs
StatePublished - Sep 5 2005

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