Proteome profiling of chicken ovarian follicles immediately before and after cyclic recruitment

Kahina Ghanem, Alan L. Johnson

Research output: Contribution to journalArticlepeer-review

Abstract

A shotgun proteomics study using isobaric tags for relative and absolute quantification labeling was conducted to characterize proteins in chicken ovarian follicles immediately before and after cyclic recruitment. Granulosa cell (GC) layers from the most recently recruited follicle (GC9) and from each of the four largest prerecruitment follicles (GC1–4) plus theca tissue (TH) from the most recently recruited (TH9) and largest prerecruitment (TH1) follicles were compared. Of 1535 proteins identified, none were determined to be differentially expressed between TH9 and TH1. A pairwise comparison between GC9 and GC1, GC2, GC3, or GC4 resulted in one, five, five, and six differentially expressed proteins, respectively, including yolk and cholesterol transport proteins (vitellogenin 1–3 and apolipoprotein B). In addition, transforming growth factor-beta 1 and microRNA-21 pathways were predicted to be activated at recruitment. We also report, for the first time, the expression of the neuropeptide, RELAXIN-3 (RLN3), in GC. Quantitative polymerase chain reaction determined RLN3 expression to be highest in GC9 and GC1, but its receptors, RXFP1 and RXFP3, were highest in TH and ovarian stroma, respectively. Overall, cyclic recruitment is associated with changes in protein expression predominantly within follicle GC, and a potential role for RLN3 in follicle recruitment and the initiation of GC differentiation warrants further investigation.

Original languageEnglish (US)
Pages (from-to)571-583
Number of pages13
JournalMolecular Reproduction and Development
Volume88
Issue number8
DOIs
StatePublished - Aug 2021

Bibliographical note

Funding Information:
We thank Anne Stanley and Dr. Bruce Stanley at Penn State College of Medicine's Mass Spectrometry & Proteomics Core Facility for making this proteomics study possible. Anne Stanley provided us with guidance in preparing the protein samples, iTRAQ labeling, running the 2D LC/MSMS, and analyzing the data. This study was supported by the National Science Foundation (IOS‐1354713), the Walther H. Ott Endowment at the Pennsylvania State University, and the USDA National Institute of Food and Agriculture Federal Appropriations [Project PEN04580 and Accession number 1005469] to Alan L. Johnson.

Funding Information:
We thank Anne Stanley and Dr. Bruce Stanley at Penn State College of Medicine's Mass Spectrometry & Proteomics Core Facility for making this proteomics study possible. Anne Stanley provided us with guidance in preparing the protein samples, iTRAQ labeling, running the 2D LC/MSMS, and analyzing the data. This study was supported by the National Science Foundation (IOS-1354713), the Walther H. Ott Endowment at the Pennsylvania State University, and the USDA National Institute of Food and Agriculture Federal Appropriations [Project PEN04580 and Accession number 1005469] to Alan L. Johnson.

Publisher Copyright:
© 2021 Wiley Periodicals LLC

Keywords

  • chicken
  • cyclic recruitment
  • follicle
  • ovary
  • proteomics

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

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