TY - JOUR
T1 - Prothrombin fragment 1-membrane interactions
T2 - a calcium-43 NMR study
AU - Bouhoutsos-Brown, Elene
AU - Pletcher, Carol H.
AU - Nelsestuen, Gary L.
AU - Bryant, Robert G.
PY - 1984/8
Y1 - 1984/8
N2 - The 43Ca NMR spectra are reported for solutions of prothrombin fragment 1 in the presence and absence of phospholipid. The calcium NMR spectrum permits distinction between three thermodynamic classes of calcium-binding interactions. The calcium ion in the lipid-free solutions was labile, with maximum residence times estimated for the average protein site in the range of 0.5-1 ms. The calcium spectrum was sensitive to the protein association and the addition of phospholipid, which appears to sharpen the calcium specificity for the protein sites. The calcium NMR spectra in the presence of phospholipid are similar to those in lipid-free solutions, which suggests that the calcium ion remains labile in the lipid-protein complex.
AB - The 43Ca NMR spectra are reported for solutions of prothrombin fragment 1 in the presence and absence of phospholipid. The calcium NMR spectrum permits distinction between three thermodynamic classes of calcium-binding interactions. The calcium ion in the lipid-free solutions was labile, with maximum residence times estimated for the average protein site in the range of 0.5-1 ms. The calcium spectrum was sensitive to the protein association and the addition of phospholipid, which appears to sharpen the calcium specificity for the protein sites. The calcium NMR spectra in the presence of phospholipid are similar to those in lipid-free solutions, which suggests that the calcium ion remains labile in the lipid-protein complex.
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U2 - 10.1016/0162-0134(84)85055-2
DO - 10.1016/0162-0134(84)85055-2
M3 - Article
C2 - 6548252
AN - SCOPUS:0021171131
VL - 21
SP - 337
EP - 343
JO - Journal of Inorganic Biochemistry
JF - Journal of Inorganic Biochemistry
SN - 0162-0134
IS - 4
ER -