TY - JOUR
T1 - Pseudogene PTENP1 functions as a competing endogenous RNA to suppress clear-cell renal cell carcinoma progression
AU - Yu, Gan
AU - Yao, Weimin
AU - Gumireddy, Kiranmai
AU - Li, Anping
AU - Wang, Ji
AU - Xiao, Wei
AU - Chen, Ke
AU - Xiao, Haibing
AU - Li, Heng
AU - Tang, Kun
AU - Ye, Zhangqun
AU - Huang, Qihong
AU - Xu, Hua
N1 - Publisher Copyright:
©2014 AACR.
PY - 2014/12/1
Y1 - 2014/12/1
N2 - PTENP1 is a pseudogene of the PTEN tumor suppression gene (TSG). The functions of PTENP1 in clearcell renal cell carcinoma (ccRCC) have not yet been studied. We found that PTENP1 is downregulated in ccRCC tissues and cells due to methylation. PTENP1 and PTEN are direct targets of miRNA miR21 and their expression is suppressed by miR21 in ccRCC cell lines. miR21 expression promotes ccRCC cell proliferation, migration, invasion in vitro, and tumor growth and metastasis in vivo . Overexpression of PTENP1 in cells expressing miR21 reduces cell proliferation, invasion, tumor growth, and metastasis, recapitulating the phenotypes induced by PTEN expression. Overexpression of PTENP1 in ccRCC cells sensitizes these cells to cisplatin and gemcitabine treatments in vitro and in vivo. In clinical samples, the expression of PTENP1 and PTEN is correlated, and both expressions are inversely correlated with miR21 expression. Patients with ccRCC with no PTENP1 expression have a lower survival rate. These results suggest that PTENP1 functions as a competing endogenous RNA (ceRNA) in ccRCC to suppress cancer progression.
AB - PTENP1 is a pseudogene of the PTEN tumor suppression gene (TSG). The functions of PTENP1 in clearcell renal cell carcinoma (ccRCC) have not yet been studied. We found that PTENP1 is downregulated in ccRCC tissues and cells due to methylation. PTENP1 and PTEN are direct targets of miRNA miR21 and their expression is suppressed by miR21 in ccRCC cell lines. miR21 expression promotes ccRCC cell proliferation, migration, invasion in vitro, and tumor growth and metastasis in vivo . Overexpression of PTENP1 in cells expressing miR21 reduces cell proliferation, invasion, tumor growth, and metastasis, recapitulating the phenotypes induced by PTEN expression. Overexpression of PTENP1 in ccRCC cells sensitizes these cells to cisplatin and gemcitabine treatments in vitro and in vivo. In clinical samples, the expression of PTENP1 and PTEN is correlated, and both expressions are inversely correlated with miR21 expression. Patients with ccRCC with no PTENP1 expression have a lower survival rate. These results suggest that PTENP1 functions as a competing endogenous RNA (ceRNA) in ccRCC to suppress cancer progression.
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U2 - 10.1158/1535-7163.MCT-14-0245
DO - 10.1158/1535-7163.MCT-14-0245
M3 - Article
AN - SCOPUS:84918545890
SN - 1535-7163
VL - 13
SP - 3086
EP - 3097
JO - Molecular Cancer Therapeutics
JF - Molecular Cancer Therapeutics
IS - 12
ER -
