Abstract
A simple, rapid plasmid mini-prep procedure for lactococci and lactobacilli which gives high yields and can be performed on overnight broth cultures is presented. Large plasmids were isolated from both lactococci and lactobacilli, including a 70-kb plasmid from Lactobacillus acidophilus C7. The purity of the resulting plasmid DNA makes it suitable for subsequent molecular manipulations. The convenience of the technique makes this rapid mini-prep procedure suitable for routine plasmid isolation from lactic acid bacteria.
Original language | English (US) |
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Pages (from-to) | 2730-2733 |
Number of pages | 4 |
Journal | Applied and environmental microbiology |
Volume | 59 |
Issue number | 8 |
DOIs |
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State | Published - 1993 |