TY - JOUR
T1 - Receptor-mediated generation of an EDRF-like intermediate in a neuronal cell line detected by spin trapping techniques
AU - Arroyo, Carmen M.
AU - Forray, Carlos
AU - El-Fakahany, Esam E.
AU - Rosen, Gerald M.
N1 - Funding Information:
This researchw as supported by grants from NIH, (G.M.R.), NIH and U.S. Army ResearchO ffice (E.E.E.).
PY - 1990/8/16
Y1 - 1990/8/16
N2 - We have studied receptor-mediated generation of an activator of soluble guanylate cyclase in cultured mouse neuroblastoma cells (clone N1E-115) by ESR/spin trapping spectroscopy. A spin adduct was detected during the activation of muscarinic receptors by carbamylcholine in the presence of the spin trap 3,5-dibromo 4-nitrosobenzene sulphonate (DBNBS). The spin adduct does not correspond to that originating from the free radical nitric oxide or hydroxylamine. The same adduct was generated in cytosol preparations from N1E-115 cells incubated with L-arginine, NADPH, in the presence of calcium. The use of isotopically labelled guanidino-N15-L-arginine supported the generation of a DBNBS spin trapped adduct originating from the guanidino moiety of L-arginine. Superoxide dismutase (SOD) stabilized the precursor of the spin adduct as well as the activator of soluble guanylate cyclase derived from L-arginine. Our results provide direct evidence for the receptor-mediated formation of a diffusible precursor of NO{up triangle, filled} derived from L-arginine.
AB - We have studied receptor-mediated generation of an activator of soluble guanylate cyclase in cultured mouse neuroblastoma cells (clone N1E-115) by ESR/spin trapping spectroscopy. A spin adduct was detected during the activation of muscarinic receptors by carbamylcholine in the presence of the spin trap 3,5-dibromo 4-nitrosobenzene sulphonate (DBNBS). The spin adduct does not correspond to that originating from the free radical nitric oxide or hydroxylamine. The same adduct was generated in cytosol preparations from N1E-115 cells incubated with L-arginine, NADPH, in the presence of calcium. The use of isotopically labelled guanidino-N15-L-arginine supported the generation of a DBNBS spin trapped adduct originating from the guanidino moiety of L-arginine. Superoxide dismutase (SOD) stabilized the precursor of the spin adduct as well as the activator of soluble guanylate cyclase derived from L-arginine. Our results provide direct evidence for the receptor-mediated formation of a diffusible precursor of NO{up triangle, filled} derived from L-arginine.
UR - http://www.scopus.com/inward/record.url?scp=0025006809&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025006809&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(90)90517-Q
DO - 10.1016/0006-291X(90)90517-Q
M3 - Article
C2 - 1975169
AN - SCOPUS:0025006809
SN - 0006-291X
VL - 170
SP - 1177
EP - 1183
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -