Recovery of YAC-end sequences through complementation of an Escherichia coli pyrF mutation

David A. Wright; Sei Kyoung Park; Dongying Wu; Gregory J. Phillips; Steven R. Rodermel; Daniel F. Voytas

doi:10.1093/nar/25.13.2679

Recovery of YAC-end sequences through complementation of an Escherichia coli pyrF mutation

David A. Wright, Sei Kyoung Park, Dongying Wu, Gregory J. Phillips, Steven R. Rodermel, Daniel F. Voytas

Genetics, Cell Biology and Development (CBS)

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Abstract

We have developed a genetic means to recover sequences from YAC-ends near the yeast selectable marker URA3. This strategy is based on the ability of URA3 to complement mutations in pyrF, an Escherichia coli gene required for pyrimidine biosynthesis. We have developed an E. coli strain with a non-reverting allele of pyrF that is also suitable for cloning (recA^-, hsdR^-). We demonstrate the utility of this complementation strategy to obtain right-end clones from three YACs containing Arabidopsis thaliana DNA.

Original language	English (US)
Pages (from-to)	2679-2680
Number of pages	2
Journal	Nucleic acids research
Volume	25
Issue number	13
DOIs	https://doi.org/10.1093/nar/25.13.2679
State	Published - Jul 1 1997

Bibliographical note

Funding Information:
This work was supported by DOE grant DE-FG02-94ER20147. This is Journal Paper No. J-17307 of the Iowa Agriculture and Home Economics Experiment Station, Ames, IA. Project No. 3120, and supported by Hatch Act and State of Iowa funds.

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abstract = "We have developed a genetic means to recover sequences from YAC-ends near the yeast selectable marker URA3. This strategy is based on the ability of URA3 to complement mutations in pyrF, an Escherichia coli gene required for pyrimidine biosynthesis. We have developed an E. coli strain with a non-reverting allele of pyrF that is also suitable for cloning (recA-, hsdR-). We demonstrate the utility of this complementation strategy to obtain right-end clones from three YACs containing Arabidopsis thaliana DNA.",

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AU - Phillips, Gregory J.

AU - Rodermel, Steven R.

AU - Voytas, Daniel F.

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Recovery of YAC-end sequences through complementation of an Escherichia coli pyrF mutation

Abstract

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