Relative expression of the products of glyoxylate bypass operon: Contributions of transcription and translation

T. Chung, E. Resnik, C. Stueland, D. C. LaPorte

Research output: Contribution to journalComment/debatepeer-review

10 Scopus citations

Abstract

Although the genes of the aceBAK operon are expressed from the same promoter, the relative cellular levels of their products are approximately 0.3:1:0.003. Gene and operon fusions with lacZ were constructed to characterize this differential expression. The upshift in expression between aceB and aceA resulted from differences in translational efficiency. In contrast, inefficient translation and premature transcriptional termination contributed to the downshift in expression between aceA and aceK. Premature transcriptional termination occurred within aceK and appears to result from inefficient translation. Deletion of repetitive extragenic palindromic elements between aceA and aceK had little effect on the relative expression of these genes. Rather, the sequences responsible for inefficient expression of aceK lie within the aceK ribosome binding site.

Original languageEnglish (US)
Pages (from-to)4572-4575
Number of pages4
JournalJournal of bacteriology
Volume175
Issue number14
DOIs
StatePublished - 1993

Fingerprint

Dive into the research topics of 'Relative expression of the products of glyoxylate bypass operon: Contributions of transcription and translation'. Together they form a unique fingerprint.

Cite this