Abstract
In an earlier paper [35], we examined the mutual interaction between the actin cytoskeleton and the cell membrane and explored the role this interaction plays during freeze/thaw. In this follow-up paper, we investigate the physical and chemical stresses induced by freeze/thaw and explore the different mechanisms of damage caused by these stresses. Our results showed that changes in cell volume during freeze/thaw and the unfrozen water content in the solution alter the cytoskeleton stiffness, and the available membrane material. Combined with unfavorable ice-membrane interactions and increasing membrane stiffness, increased de-structuring of the membrane (such as bleb and microvilli formation) synergistically act on the membrane-cytoskeleton system generating irreversible damage.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 96-104 |
| Number of pages | 9 |
| Journal | Cryobiology |
| Volume | 66 |
| Issue number | 2 |
| DOIs | |
| State | Published - Apr 2013 |
| Externally published | Yes |
Bibliographical note
Funding Information:Statement of funding: This research was partially supported by a University of Minnesota Doctoral Dissertation Fellowship awarded to V.R. and the University of Minnesota McKnight Land-Grant Professorship awarded to A.A. as well as an NSF Grant ( CBET-0644784 ) and its REU supplement.
Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
Keywords
- Actin cytoskeleton
- Cryopreservation
- Freeze/thaw
- Membrane