TY - JOUR
T1 - Rickettsia buchneri sp. Nov., a rickettsial endosymbiont of the blacklegged tick ixodes scapularis
AU - Kurtti, Timothy J.
AU - Felsheim, Roderick F.
AU - Burkhardt, Nicole Y.
AU - Oliver, Jonathan D.
AU - Heu, Chan C.
AU - Munderloh, Ulrike G.
N1 - Publisher Copyright:
© 2015 IUMS.
PY - 2015
Y1 - 2015
N2 - We obtained a rickettsial isolate from the ovaries of the blacklegged tick, Ixodes scapularis. The isolate (ISO7T) was grown in the Ixodes ricinus embryonic cell line IRE11. We characterized the isolate by transmission electron microscopy and gene sequencing. Phylogenetic analysis of 11 housekeeping genes demonstrated that the isolate fulfils the criteria to be classified as a representative of a novel rickettsial species closely related to ‘Rickettsia monacensis’. These rickettsiae form a clade separate from other species of rickettsiae. Gene sequences indicated that several genes important in rickettsial motility, invasiveness and temperature adaptation were mutated (e.g. sca2, rickA, hsp22, pldA and htrA). We propose the name Rickettsia buchneri sp. nov. for this bacterium that infects the ovaries of the tick I. scapularis to acknowledge the pioneering contributions of Professor Paul Buchner (1886-1978) to research on bacterial symbionts. The type strain of R. buchneri sp. nov.
AB - We obtained a rickettsial isolate from the ovaries of the blacklegged tick, Ixodes scapularis. The isolate (ISO7T) was grown in the Ixodes ricinus embryonic cell line IRE11. We characterized the isolate by transmission electron microscopy and gene sequencing. Phylogenetic analysis of 11 housekeeping genes demonstrated that the isolate fulfils the criteria to be classified as a representative of a novel rickettsial species closely related to ‘Rickettsia monacensis’. These rickettsiae form a clade separate from other species of rickettsiae. Gene sequences indicated that several genes important in rickettsial motility, invasiveness and temperature adaptation were mutated (e.g. sca2, rickA, hsp22, pldA and htrA). We propose the name Rickettsia buchneri sp. nov. for this bacterium that infects the ovaries of the tick I. scapularis to acknowledge the pioneering contributions of Professor Paul Buchner (1886-1978) to research on bacterial symbionts. The type strain of R. buchneri sp. nov.
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U2 - 10.1099/ijs.0.000047
DO - 10.1099/ijs.0.000047
M3 - Article
C2 - 25563918
AN - SCOPUS:84925008974
SN - 1466-5026
VL - 65
SP - 965
EP - 970
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 3
ER -