Role of cardiac sympathetic nerves in blood pressure regulation

Erica A. Wehrwein; Misa Yoshimoto; Pilar Guzman; Amit Shah; David L. Kreulen; John W. Osborn

doi:10.1016/j.autneu.2014.02.005

Role of cardiac sympathetic nerves in blood pressure regulation

Erica A. Wehrwein, Misa Yoshimoto, Pilar Guzman, Amit Shah, David L. Kreulen, John W. Osborn

Surgery

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Abstract

Stellate ganglionectomy (SGx) was used to assess the contribution of cardiac sympathetic nerves to neurogenic hypertension in deoxycorticosterone (DOCA)-salt treated rats. Experiments were conducted in two substrains of Sprague-Dawley (SD) rats since previous studies reported bradycardia in Charles River-SD (CR-SD) rats and tachycardia in SASCO-SD (SA-SD) rats with DOCA treatment suggesting different underlying neural mechanisms. Uninephrectomized male rats underwent SGx or SHAM surgery and were instrumented for telemetric monitoring of mean arterial pressure (MAP) and heart rate (HR). After recovery, 0.9% saline solution and DOCA (50. mg) were administered. Baseline MAP (Days 0-5 average) after SGx in CR-SD rats (96. ±. 2. mm. Hg; n. = 7) was not significantly different (p. = 0.08) than CR-SD SHAM rats (103. ±. 3. mm. Hg; n. = 9); however, there was a significantly lower HR during the baseline period (377. ±. 7 vs. 432. ±. 7. bpm, p. <. 0.05) in SGx rats. In SA-SD rats baseline MAP was not different between SGx and SHAM rats and HR was lower in SGx rats (428. ±. 8 vs. 371. ±. 5. bpm, p. <. 0.05). After DOCA treatment in both substrains, MAP and HR were elevated similarly in SHAM and SGx groups showing minimal impact in both groups of SGx on hypertension development. However, overall MAP in SA-SD SHAM rats reached a significantly higher level (155. ±. 10. mm. Hg vs 135. ±. 5. mm. Hg, p. <. 0.05) than that observed in CR-SD SHAM rats demonstrating that the magnitude of hypertensive response to DOCA-salt treatment varies between substrains. In conclusion, removal of cardiac sympathetic nerves did not alter the development or maintenance of DOCA-salt hypertension in SD rats.

Original language	English (US)
Pages (from-to)	30-35
Number of pages	6
Journal	Autonomic Neuroscience: Basic and Clinical
Volume	183
DOIs	https://doi.org/10.1016/j.autneu.2014.02.005
State	Published - Jul 2014

Bibliographical note

Funding Information:
Funding was provided by the American Heart Association Predoctoral Fellowship (EAW), NIH PO1HL70687 (DLK), and NHLBI R01 HL64176 (JWO).

Keywords

Catecholamines
Ganglionectomy
Heart
Stellate ganglion

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@article{9107aa58ea3c4832821d740180e7e691,

title = "Role of cardiac sympathetic nerves in blood pressure regulation",

abstract = "Stellate ganglionectomy (SGx) was used to assess the contribution of cardiac sympathetic nerves to neurogenic hypertension in deoxycorticosterone (DOCA)-salt treated rats. Experiments were conducted in two substrains of Sprague-Dawley (SD) rats since previous studies reported bradycardia in Charles River-SD (CR-SD) rats and tachycardia in SASCO-SD (SA-SD) rats with DOCA treatment suggesting different underlying neural mechanisms. Uninephrectomized male rats underwent SGx or SHAM surgery and were instrumented for telemetric monitoring of mean arterial pressure (MAP) and heart rate (HR). After recovery, 0.9% saline solution and DOCA (50. mg) were administered. Baseline MAP (Days 0-5 average) after SGx in CR-SD rats (96. ±. 2. mm. Hg; n. = 7) was not significantly different (p. = 0.08) than CR-SD SHAM rats (103. ±. 3. mm. Hg; n. = 9); however, there was a significantly lower HR during the baseline period (377. ±. 7 vs. 432. ±. 7. bpm, p. <. 0.05) in SGx rats. In SA-SD rats baseline MAP was not different between SGx and SHAM rats and HR was lower in SGx rats (428. ±. 8 vs. 371. ±. 5. bpm, p. <. 0.05). After DOCA treatment in both substrains, MAP and HR were elevated similarly in SHAM and SGx groups showing minimal impact in both groups of SGx on hypertension development. However, overall MAP in SA-SD SHAM rats reached a significantly higher level (155. ±. 10. mm. Hg vs 135. ±. 5. mm. Hg, p. <. 0.05) than that observed in CR-SD SHAM rats demonstrating that the magnitude of hypertensive response to DOCA-salt treatment varies between substrains. In conclusion, removal of cardiac sympathetic nerves did not alter the development or maintenance of DOCA-salt hypertension in SD rats.",

keywords = "Catecholamines, Ganglionectomy, Heart, Stellate ganglion",

author = "Wehrwein, {Erica A.} and Misa Yoshimoto and Pilar Guzman and Amit Shah and Kreulen, {David L.} and Osborn, {John W.}",

note = "Funding Information: Funding was provided by the American Heart Association Predoctoral Fellowship (EAW), NIH PO1HL70687 (DLK), and NHLBI R01 HL64176 (JWO). ",

year = "2014",

month = jul,

doi = "10.1016/j.autneu.2014.02.005",

language = "English (US)",

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journal = "Autonomic Neuroscience: Basic and Clinical",

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T1 - Role of cardiac sympathetic nerves in blood pressure regulation

AU - Wehrwein, Erica A.

AU - Yoshimoto, Misa

AU - Guzman, Pilar

AU - Shah, Amit

AU - Kreulen, David L.

AU - Osborn, John W.

N1 - Funding Information: Funding was provided by the American Heart Association Predoctoral Fellowship (EAW), NIH PO1HL70687 (DLK), and NHLBI R01 HL64176 (JWO).

PY - 2014/7

Y1 - 2014/7

N2 - Stellate ganglionectomy (SGx) was used to assess the contribution of cardiac sympathetic nerves to neurogenic hypertension in deoxycorticosterone (DOCA)-salt treated rats. Experiments were conducted in two substrains of Sprague-Dawley (SD) rats since previous studies reported bradycardia in Charles River-SD (CR-SD) rats and tachycardia in SASCO-SD (SA-SD) rats with DOCA treatment suggesting different underlying neural mechanisms. Uninephrectomized male rats underwent SGx or SHAM surgery and were instrumented for telemetric monitoring of mean arterial pressure (MAP) and heart rate (HR). After recovery, 0.9% saline solution and DOCA (50. mg) were administered. Baseline MAP (Days 0-5 average) after SGx in CR-SD rats (96. ±. 2. mm. Hg; n. = 7) was not significantly different (p. = 0.08) than CR-SD SHAM rats (103. ±. 3. mm. Hg; n. = 9); however, there was a significantly lower HR during the baseline period (377. ±. 7 vs. 432. ±. 7. bpm, p. <. 0.05) in SGx rats. In SA-SD rats baseline MAP was not different between SGx and SHAM rats and HR was lower in SGx rats (428. ±. 8 vs. 371. ±. 5. bpm, p. <. 0.05). After DOCA treatment in both substrains, MAP and HR were elevated similarly in SHAM and SGx groups showing minimal impact in both groups of SGx on hypertension development. However, overall MAP in SA-SD SHAM rats reached a significantly higher level (155. ±. 10. mm. Hg vs 135. ±. 5. mm. Hg, p. <. 0.05) than that observed in CR-SD SHAM rats demonstrating that the magnitude of hypertensive response to DOCA-salt treatment varies between substrains. In conclusion, removal of cardiac sympathetic nerves did not alter the development or maintenance of DOCA-salt hypertension in SD rats.

AB - Stellate ganglionectomy (SGx) was used to assess the contribution of cardiac sympathetic nerves to neurogenic hypertension in deoxycorticosterone (DOCA)-salt treated rats. Experiments were conducted in two substrains of Sprague-Dawley (SD) rats since previous studies reported bradycardia in Charles River-SD (CR-SD) rats and tachycardia in SASCO-SD (SA-SD) rats with DOCA treatment suggesting different underlying neural mechanisms. Uninephrectomized male rats underwent SGx or SHAM surgery and were instrumented for telemetric monitoring of mean arterial pressure (MAP) and heart rate (HR). After recovery, 0.9% saline solution and DOCA (50. mg) were administered. Baseline MAP (Days 0-5 average) after SGx in CR-SD rats (96. ±. 2. mm. Hg; n. = 7) was not significantly different (p. = 0.08) than CR-SD SHAM rats (103. ±. 3. mm. Hg; n. = 9); however, there was a significantly lower HR during the baseline period (377. ±. 7 vs. 432. ±. 7. bpm, p. <. 0.05) in SGx rats. In SA-SD rats baseline MAP was not different between SGx and SHAM rats and HR was lower in SGx rats (428. ±. 8 vs. 371. ±. 5. bpm, p. <. 0.05). After DOCA treatment in both substrains, MAP and HR were elevated similarly in SHAM and SGx groups showing minimal impact in both groups of SGx on hypertension development. However, overall MAP in SA-SD SHAM rats reached a significantly higher level (155. ±. 10. mm. Hg vs 135. ±. 5. mm. Hg, p. <. 0.05) than that observed in CR-SD SHAM rats demonstrating that the magnitude of hypertensive response to DOCA-salt treatment varies between substrains. In conclusion, removal of cardiac sympathetic nerves did not alter the development or maintenance of DOCA-salt hypertension in SD rats.

KW - Catecholamines

KW - Ganglionectomy

KW - Heart

KW - Stellate ganglion

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JO - Autonomic Neuroscience: Basic and Clinical

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ER -

Role of cardiac sympathetic nerves in blood pressure regulation

Abstract

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Keywords

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