Saccharomyces cerevisiae cells secreting an aspergillus higer β-galactosidase grow on whey permeate

Vijay Kumar; Sundaram Ramakrishnan; Tuula T. Teeri; Jonathan K.C. Knowles; Brian S. Hartley

doi:10.1038/nbt0192-82

Saccharomyces cerevisiae cells secreting an aspergillus higer β-galactosidase grow on whey permeate

Vijay Kumar, Sundaram Ramakrishnan, Tuula T. Teeri, Jonathan K.C. Knowles, Brian S. Hartley

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Abstract

We describe the construction of a lactose- utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermostable β-galactosidase (lacA) from Aspergillus niger. Yeast cells expressing the lacA gene from the yeast ADH1 promoter on a multicopy plasmid secrete up to 40% of the total β-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the lacA gene grow on whey permeate (4% w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.

Original language	English (US)
Pages (from-to)	82-85
Number of pages	4
Journal	Bio/Technology
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/nbt0192-82
State	Published - Jan 1992
Externally published	Yes

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AU - Hartley, Brian S.

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AB - We describe the construction of a lactose- utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermostable β-galactosidase (lacA) from Aspergillus niger. Yeast cells expressing the lacA gene from the yeast ADH1 promoter on a multicopy plasmid secrete up to 40% of the total β-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the lacA gene grow on whey permeate (4% w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.

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Saccharomyces cerevisiae cells secreting an aspergillus higer β-galactosidase grow on whey permeate

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